ArticleBMC systems biology2013
The last generation of bacterial growth in limiting nutrient.
Article in BMC systems biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- Analysing and meta-analysing time-series data of microbial growth and gene expression from plate readers.PLoS computational biology · 2022Pooled it
- Identifiability, Sensitivity, and Genetic Algorithms in Bacterial Biofilm Selection Models.Bulletin of mathematical biology · 2026Article
- Growth-density inversion in Escherichia coli reveals superlinear, not sublinear, density dependence.PLoS biology · 2026Article
- Growth control as a central regulator for tuning the cellular context.Journal of biological engineering · 2026Article
- Maltose aids in the growth ofFEMS microbes · 2026Article
- The impact of human milk oligosaccharides and galacto-oligosaccharides on the growth of early life pathogenic gut bacteria.Frontiers in microbiology · 2026Article
- Article
- Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.The EMBO journal · 2025Article
- Principles of bacteriostatic and bactericidal antibiotics at subinhibitory concentrations.mBio · 2025Article
- CcpA-mediated regulation of cellular energy metabolism in the ruminal bacteriumMicrobiology spectrum · 2025Article
- Developing Lotka-Volterra Based Models to Describe Bdellovibrio Predation in a Batch and Chemostat Experimental System.Environmental microbiology reports · 2025Article
- Glycogen phase separation drives macromolecular rearrangement and asymmetric division inbioRxiv : the preprint server for biology · 2025Article
- Community-specific cell death sustains bacterial expansion under phosphorus starvation.Nature chemical biology · 2025Article
- Incoherent feedback from coupled amino acids and ribosome pools generates damped oscillations in growing E. coli.Nature communications · 2025Article
- Data-driven discovery of the interplay between genetic and environmental factors in bacterial growth.Communications biology · 2024Article
- Nutrient colimitation is a quantitative, dynamic property of microbial populations.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- PHB production by Bacillus megaterium strain MNSH1-9K-1 using low-cost media.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- In vitro fermentation potential of gut endogenous protein losses of growing pigs.Journal of animal science · 2024Article
- The Prospect of Hydrolytic Enzymes fromInternational journal of molecular sciences · 2023Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBacterial growth as a function of nutrients has been studied for decades, but is still not fully understood. In particular, the growth laws under dynamically changing environments have been difficult to explore, because of the rapidly changing conditions. Here, we address this challenge by means of a robotic assay and measure bacterial growth rate, promoter activity and substrate level at high temporal resolution across the entire growth curve in batch culture. As a model system, we study E. coli growing under nitrogen or carbon limitation, and explore the dynamics in the last generation of growth where nutrient levels can drop rapidly.
resultsWe find that growth stops abruptly under limiting nitrogen or carbon, but slows gradually when nutrients are not limiting. By measuring growth rate at a 3 min time resolution, and inferring the instantaneous substrate level, s, we find that the reduction in growth rate μ under nutrient limitation follows Monod's law, μ=μ0(s/(k(s)+s)). By following promoter activity of different genes we found that the abrupt stop of growth under nitrogen or carbon limitation is accompanied by a pulse-like up-regulation of the expression of genes in the relevant nutrient assimilation pathways. We further find that sharp stop of growth is conditional on the presence of regulatory proteins in the assimilation pathway.
conclusionsThe observed sharp stop of growth accompanied by a pulsed expression of assimilation genes allows bacteria to compensate for the drop in nutrients, suggesting a strategy used by the cells to prolong exponential growth under limiting substrate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.