ArticleBMC biotechnology2013
Enhanced transgene expression in rice following selection controlled by weak promoters.
Article in BMC biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 29 citations in OpenAlex.
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- A Core35S Promoter of Cauliflower Mosaic Virus Drives More Efficient Replication of Turnip Crinkle Virus.Plants (Basel, Switzerland) · 2021Article
- Targeted Transgene Expression in Rice Using a Callus Strong Promoter for Selectable Marker Gene Control.Frontiers in plant science · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Rice black-streaked dwarf virus P10 acts as either a synergistic or antagonistic determinant during superinfection with related or unrelated virus.Molecular plant pathology · 2019Article
- Isolation and identification of a vegetative organ-specific promoter from maize.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Synthetic Promoters: Designing the cis Regulatory Modules for Controlled Gene Expression.Molecular biotechnology · 2018Review
- Isolation, characterization, and evaluation of three Citrus sinensis-derived constitutive gene promoters.Plant cell reports · 2018Article
- Article
- Identification of a 467 bp Promoter of Maize Phosphatidylinositol Synthase Gene (ZmPIS) Which Confers High-Level Gene Expression and Salinity or Osmotic Stress Inducibility in Transgenic Tobacco.Frontiers in plant science · 2016Article
- Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation.Plant cell reports · 2015Article
- Heterologous expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in rice leads to lower arsenic accumulation in grain.Scientific reports · 2014Article
- Specific expression of DR5 promoter in rice roots using a tCUP derived promoter-reporter system.PloS one · 2014Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTechniques that enable high levels of transgene expression in plants are attractive for the commercial production of plant-made recombinant pharmaceutical proteins or other gene transfer related strategies. The conventional way to increase the yield of desired transgenic products is to use strong promoters to control the expression of the transgene. Although many such promoters have been identified and characterized, the increase obtainable from a single promoter is ultimately limited to a certain extent.
resultsIn this study, we report a method to magnify the effect of a single promoter by using a weak promoter-based selection system in transgenic rice. tCUP1, a fragment derived from the tobacco cryptic promoter (tCUP), was tested for its activity in rice by fusion to both a β-glucuronidase (GUS) reporter and a hygromycin phosphotransferase (HPT) selectable marker. The tCUP1 promoter allowed the recovery of transformed rice plants and conferred tissue specific expression of the GUS reporter, but was much weaker than the CaMV 35S promoter in driving a selectable marker for growth of resistant calli. However, in the resistant calli and regenerated transgenic plants selected by the use of tCUP1, the constitutive expression of green fluorescent protein (GFP) was dramatically increased as a result of the additive effect of multiple T-DNA insertions. The correlation between attenuated selection by a weak promoter and elevation of copy number and foreign gene expression was confirmed by using another relatively weak promoter from nopaline synthase (Nos).
conclusionsThe use of weak promoter derived selectable markers leads to a high T-DNA copy number and then greatly increases the expression of the foreign gene. The method described here provides an effective approach to robustly enhance the expression of heterogenous transgenes through copy number manipulation in rice.
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