ArticleProceedings of the National Academy of Sciences of the United States of America2013
Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 137 citations in OpenAlex.
- A New Family of Electron-Bifurcating Enzymes Represented by Ferredoxin- and NAD-Dependent Glycerol-3-Phosphate Oxidoreductase.Biochemistry · 2026Article
- Advances in metabolic engineering and fermentation for 3-hydroxypropionic acid biosynthesis: a comprehensive review.World journal of microbiology & biotechnology · 2025Review
- Markerless mutagenesis enables isoleucine biosynthesis solely from threonine inMicrobiology spectrum · 2025Article
- Designing thermophilic, synthetic microbial communities for consolidated bioprocessing.Biodesign research · 2025Review
- Engineering the hyperthermophilic archaeonApplied and environmental microbiology · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Methanothermobacter thermautotrophicus and Alternative Methanogens: Archaea-Based Production.Advances in biochemical engineering/biotechnology · 2025Review
- Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?ChemCatChem · 2024Article
- A pH-dependent shift of redox cofactor specificity in a benzyl alcohol dehydrogenase of aromatoleum aromaticum EbN1.Applied microbiology and biotechnology · 2024Article
- Manipulating Fermentation Pathways in the Hyperthermophilic ArchaeonApplied and environmental microbiology · 2023Article
- Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus.Applied and environmental microbiology · 2023Article
- Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways.Journal of advanced research · 2023Review
- Biocatalytic conversion of sunlight and carbon dioxide to solar fuels and chemicals.RSC advances · 2022Review
- Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks.Frontiers in bioengineering and biotechnology · 2022Review
- Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients.Frontiers in bioengineering and biotechnology · 2022Review
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Metabolic engineering strategies to enable microbial utilization of C1 feedstocks.Nature chemical biology · 2021Review
- Life in hot acid: a genome-based reassessment of the archaeal order Sulfolobales.Environmental microbiology · 2021Article
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- Modification of the glycolytic pathway in Pyrococcus furiosus and the implications for metabolic engineering.Extremophiles : life under extreme conditions · 2020Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms can be engineered to produce useful products, including chemicals and fuels from sugars derived from renewable feedstocks, such as plant biomass. An alternative method is to use low potential reducing power from nonbiomass sources, such as hydrogen gas or electricity, to reduce carbon dioxide directly into products. This approach circumvents the overall low efficiency of photosynthesis and the production of sugar intermediates. Although significant advances have been made in manipulating microorganisms to produce useful products from organic substrates, engineering them to use carbon dioxide and hydrogen gas has not been reported. Herein, we describe a unique temperature-dependent approach that confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydrates at 100°C) the capacity to use carbon dioxide, a reaction that it does not accomplish naturally. This was achieved by the heterologous expression of five genes of the carbon fixation cycle of the archaeon Metallosphaera sedula, which grows autotrophically at 73°C. The engineered P. furiosus strain is able to use hydrogen gas and incorporate carbon dioxide into 3-hydroxypropionic acid, one of the top 12 industrial chemical building blocks. The reaction can be accomplished by cell-free extracts and by whole cells of the recombinant P. furiosus strain. Moreover, it is carried out some 30°C below the optimal growth temperature of the organism in conditions that support only minimal growth but maintain sufficient metabolic activity to sustain the production of 3-hydroxypropionate. The approach described here can be expanded to produce important organic chemicals, all through biological activation of carbon dioxide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.