ArticleBlood2013
Role of RNA splicing in mediating lineage-specific expression of the von Willebrand factor gene in the endothelium.
Article in Blood, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
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- Transmembrane-4 L-Six Family Member-1 Is Essential for Embryonic Blood Vessel Development.Current issues in molecular biology · 2024Article
- Multimodal Learning for Mapping the Genotype-Phenotype Dynamics.Research square · 2024Article
- Optimization of mammalian expression vector by cis-regulatory element combinations.Molecular genetics and genomics : MGG · 2023Article
- Post-transcriptional control of haemostatic genes: mechanisms and emerging therapeutic concepts in thrombo-inflammatory disorders.Cardiovascular research · 2023Review
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- Genome-wide analysis of JAZ family genes expression patterns during fig (Ficus carica L.) fruit development and in response to hormone treatment.BMC genomics · 2022Article
- Review
- A small, portable RNA device for the control of exon skipping in mammalian cells.Nucleic acids research · 2018Article
- Requirement for intron structures in activating theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation of VWF expression, and secretion in health and disease.Current opinion in hematology · 2016Review
- A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity.Nature communications · 2016Article
- FOXO1-mediated activation of Akt plays a critical role in vascular homeostasis.Circulation research · 2014Article
- Analysis of epigenetic changes in survivors of preterm birth reveals the effect of gestational age and evidence for a long term legacy.Genome medicine · 2013Article
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9 authors at 1 institution in 1 country.
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Abstract
We previously demonstrated that the first intron of the human von Willebrand factor (vWF) is required for gene expression in the endothelium of transgenic mice. Based on this finding, we hypothesized that RNA splicing plays a role in mediating vWF expression in the vasculature. To address this question, we used transient transfection assays in human endothelial cells and megakaryocytes with intron-containing and intronless human vWF promoter-luciferase constructs. Next, we generated knockin mice in which LacZ was targeted to the endogenous mouse vWF locus in the absence or presence of the native first intron or heterologous introns from the human β-globin, mouse Down syndrome critical region 1, or hagfish coagulation factor X genes. In both the in vitro assays and the knockin mice, the loss of the first intron of vWF resulted in a significant reduction of reporter gene expression in endothelial cells but not megakaryocytes. This effect was rescued to varying degrees by the introduction of a heterologous intron. Intron-mediated enhancement of expression was mediated at a posttranscriptional level. Together, these findings implicate a role for intronic splicing in mediating lineage-specific expression of vWF in the endothelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.