ArticlePlant cell reports2013
Normalizing gene expression by quantitative PCR during somatic embryogenesis in two representative conifer species: Pinus pinaster and Picea abies.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 41 citations in OpenAlex.
- Identification of best housekeeping gene for normalization in qRT-PCR data under different development stages and stress conditions in Vigna mungo.Scientific reports · 2025Article
- Temperature-induced variation in the transcriptome of maritime pine (Pinus pinaster Ait.) embryogenic masses modulates the phenotype of the derived plants.BMC genomics · 2025Article
- Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis ofFood science & nutrition · 2025Article
- Heat-Priming during Somatic Embryogenesis Increased Resilience to Drought Stress in the Generated Maritime Pine (International journal of molecular sciences · 2023Article
- Toxicity and tissue accumulation characteristics of the herbicide pendimethalin under silicon application in ginger (Zingiber officinale Roscoe).Environmental science and pollution research international · 2022Article
- MicroRNA-mediated post-transcriptional regulation of Pinus pinaster response and resistance to pinewood nematode.Scientific reports · 2022Article
- Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.Scientific reports · 2021Article
- Priming Maritime Pine Megagametophytes during Somatic Embryogenesis Improved Plant Adaptation to Heat Stress.Plants (Basel, Switzerland) · 2021Article
- Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development inFrontiers in genetics · 2021Article
- Insights Into the Mechanisms Implicated inFrontiers in plant science · 2021Article
- YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process inInternational journal of molecular sciences · 2020Article
- Article
- New Approaches to Optimize Somatic Embryogenesis in Maritime Pine.Frontiers in plant science · 2019Article
- Comprehensive assembly and analysis of the transcriptome of maritime pine developing embryos.BMC plant biology · 2018Article
- Article
- Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR.PloS one · 2016Article
- Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis.Frontiers in plant science · 2016Article
- Selection of Reference Genes for Real-Time Quantitative PCR in Pinus massoniana Post Nematode Inoculation.PloS one · 2016Article
- Article
- Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageSuitable internal control genes to normalize qPCR data from different stages of embryo development and germination were identified in two representative conifer species. Clonal propagation by somatic embryogenesis has a great application potentiality in conifers. Quantitative PCR (qPCR) is widely used for gene expression analysis during somatic embryogenesis and embryo germination. No single reference gene is universal, so a systematic characterization of endogenous genes for concrete conditions is fundamental for accuracy. We identified suitable internal control genes to normalize qPCR data obtained at different steps of somatic embryogenesis (embryonal mass proliferation, embryo maturation and germination) in two representative conifer species, Pinus pinaster and Picea abies. Candidate genes included endogenous genes commonly used in conifers, genes previously tested in model plants, and genes with a lower variation of the expression along embryo development according to genome-wide transcript profiling studies. Three different algorithms were used to evaluate expression stability. The geometric average of the expression values of elongation factor-1α, α-tubulin and histone 3 in P. pinaster, and elongation factor-1α, α-tubulin, adenosine kinase and CAC in P. abies were adequate for expression studies throughout somatic embryogenesis. However, improved accuracy was achieved when using other gene combinations in experiments with samples at a single developmental stage. The importance of studies selecting reference genes to use in different tissues or developmental stages within one or close species, and the instability of commonly used reference genes, is highlighted.
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