ArticleMethods in molecular biology (Clifton, N.J.)2013
Single-channel analysis of TRPC channels in the podocytes of freshly isolated Glomeruli.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- The effects of the atrial natriuretic peptide deficiency on renal cortical mitochondrial bioenergetics in the Dahl SS rat.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Reorganization and Suppression of Store-Operated Calcium Entry in Podocytes of Type 2 Diabetic Rats.International journal of molecular sciences · 2023Article
- Inhibition of neprilysin with sacubitril without RAS blockage aggravates renal disease in Dahl SS rats.Renal failure · 2021Article
- Insulin-activated store-operated CaNature communications · 2021Article
- Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes.International journal of molecular sciences · 2021Article
- Comprehensive assessment of mitochondrial respiratory function in freshly isolated nephron segments.American journal of physiology. Renal physiology · 2020Article
- Renal Glomerular Mitochondria Function in Salt-Sensitive Hypertension.Frontiers in physiology · 2019Article
- A NOX4/TRPC6 Pathway in Podocyte Calcium Regulation and Renal Damage in Diabetic Kidney Disease.Journal of the American Society of Nephrology : JASN · 2018Article
- Targeting the podocyte cytoskeleton: from pathogenesis to therapy in proteinuric kidney disease.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2016Review
- Chronic cathepsin inhibition by E-64 in Dahl salt-sensitive rats.Physiological reports · 2016Article
- Two-photon imaging of endothelin-1-mediated intracellular Ca(2+) handling in smooth muscle cells of rat renal resistance arteries.Life sciences · 2016Article
- Functional networks of aging markers in the glomeruli of IgA nephropathy: a new therapeutic opportunity.Oncotarget · 2016Article
- Podocyte injury in diabetic nephropathy: implications of angiotensin II-dependent activation of TRPC channels.Scientific reports · 2015Article
- Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney.Journal of visualized experiments : JoVE · 2015Article
- Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium.Journal of visualized experiments : JoVE · 2015Article
- TRPC6 channel as an emerging determinant of the podocyte injury susceptibility in kidney diseases.American journal of physiology. Renal physiology · 2015Review
- Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli.Journal of visualized experiments : JoVE · 2015Article
- Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli.Kidney international · 2014Article
- Pharmacological characterization of the P2 receptors profile in the podocytes of the freshly isolated rat glomeruli.American journal of physiology. Cell physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
One of the most important functions of the kidney is the filtration of the blood that takes place in the glomeruli. Glomerular epithelial cells (podocytes) have several functions, including regulation of the filtration process and glomerular basement membrane turnover. Dysfunction of podocytes is a major cause of glomerular kidney diseases. Gain-of-function mutations in the TRPC6 channel underlie a subset of familial forms of focal segmental glomerulosclerosis (FSGS). While growing evidence supports an important role of TRPC channels in podocytes, the regulation of these channels has yet to be investigated in freshly isolated glomeruli. Native settings in glomeruli provide, by all means, the most appropriate as well as one of the most challenging environments to study ion channel regulation. Thus, it is important to develop new methods that would better reflect the native settings of the podocytes. To address this question, we have established an experimental approach that allows studying podocytes in the freshly isolated decapsulated intact glomeruli. Here we describe the preparation of the rat glomeruli for patch-clamping, focusing on special conditions required for single-channel analysis of TRPC channels. Several tricks useful for cell-attached patch-clamping of the glomerular podocytes and solutions appropriate for registration of the TRPC channels are also provided.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.