ArticleMethods in molecular biology (Clifton, N.J.)2013
Recording ion channels in isolated, split-opened tubules.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- ENaC phosphorylation facilitates ankyrin-3 interaction critical for renal sodium balance.PNAS nexus · 2026Article
- A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport Mechanisms.Kidney360 · 2025Article
- Inhibition of mTORC2 promotes natriuresis in Dahl salt-sensitive rats via the decrease of NCC and ENaC activity.American journal of physiology. Renal physiology · 2024Article
- Proteomic analysis of murine kidney proximal tubule sub-segment derived cell lines reveals preferences in mitochondrial pathway activity.Journal of proteomics · 2023Article
- P2Y2 receptor decreases blood pressure by inhibiting ENaC.JCI insight · 2023Article
- The transcription factor Foxi1 promotes expression of V-ATPase and Gpr116 in M-1 cells.American journal of physiology. Renal physiology · 2023Article
- Bile acids regulate the epithelial NaThe Journal of physiology · 2022Article
- Functional characterization of ion channels expressed in kidney organoids derived from human induced pluripotent stem cells.American journal of physiology. Renal physiology · 2022Article
- ClC-K2 ClFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Epac1-/- and Epac2-/- mice exhibit deficient epithelial Na+ channel regulation and impaired urinary Na+ conservation.JCI insight · 2022Article
- Mechanisms and consequences of casein kinase II and ankyrin-3 regulation of the epithelial NaScientific reports · 2021Article
- With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron.Cells · 2021Article
- Stimulation of the Epithelial NaFrontiers in physiology · 2021Article
- PF-06869206 is a selective inhibitor of renal PAmerican journal of physiology. Renal physiology · 2020Article
- Article
- Vibrodissociation method for isolation of defined nephron segments from human and rodent kidneys.American journal of physiology. Renal physiology · 2019Article
- TRPV4 deletion protects against hypokalemia during systemic KAmerican journal of physiology. Renal physiology · 2019Article
- Renal NaAmerican journal of physiology. Renal physiology · 2019Article
- TRPC3 determines osmosensitive [Ca2+]i signaling in the collecting duct and contributes to urinary concentration.PloS one · 2019Article
- Physiological regulation of the epithelial NaAmerican journal of physiology. Renal physiology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Ion channels play key roles in physiology. They function as protein transducers able to transform stimuli and chemical gradients into electrical signals. They also are critical for cell signaling and play a particularly important role in epithelial transport acting as gateways for the movement of electrolytes across epithelial cell membranes. Experimental limitations, though, have hampered the recording of ion channel activity in many types of tissue. This has slowed progress in understanding the cellular and physiological function of these channels with most function inferred from in vitro systems and cell culture models. In many cases, such inferences have clouded rather than clarified the picture. Here, we describe a contemporary method for isolating and patch-clamping renal tubules for ex vivo analysis of ion channel function in native tissue. Focus is placed on quantifying the activity of the epithelial Na(+) channel (ENaC) in the aldosterone--sensitive distal nephron (ASDN). This isolated, split-open tubule preparation enables recording of renal ion channels in the close-to-native environment under the control of native cell signaling pathways and receptors. When combined with complementary measurements of organ and system function, and contemporary molecular genetics and pharmacology used to manipulate function and regulation, patch-clamping renal channels in the isolated, split-open tubule enables understanding to emerge about the physiological function of these key proteins from the molecule to the whole animal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.