ArticleProceedings of the National Academy of Sciences of the United States of America1990
Epithelial K channel expressed in Xenopus oocytes is inactivated by protein kinase C.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 13 citations in OpenAlex.
- Regulation of an inwardly rectifying K channel in the T84 epithelial cell line by calcium, nucleotides and kinases.The Journal of membrane biology · 1994Article
- Histamine modulates three types of K+ current in a human intestinal epithelial cell line.Pflugers Archiv : European journal of physiology · 1994Article
- Regulation by second messengers of the slowly activating, voltage-dependent potassium current expressed in Xenopus oocytes.The Journal of physiology · 1992Article
- Cyclic GMP regulation of a voltage-activated K channel in dissociated enterocytes.The Journal of membrane biology · 1991Article
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4 authors at 1 institution in 1 country.
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Abstract
K homeostasis is maintained in higher animals by epithelia of the kidney and intestine. Little is known regarding the molecular regulation of K secretion. We injected Xenopus oocytes with mRNA from teleost intestine, a K-secreting epithelium with apical membrane K channels. Oocytes expressed a conductance that displayed whole-cell current properties with the following characteristics: marked selectivity for K over Na and Cl, voltage-independent kinetics, Ca insensitivity, tonic activation, and inward rectification in symmetrical K. Barium, quinine, and tetraethylammonium blocked the conductance, whereas apamin, charybdotoxin, and 4-aminopyridine did not. The K conductance was rapidly (t1/2 = 10 min) and completely inactivated by 4 beta-phorbol 12-myristate 13-acetate but not by 4 alpha-phorbol 12,13-didecanoate. Sucrose density gradient fractionation revealed that mRNA required for expression is in the 1- to 2-kilobase size range, suggesting the possibility that a single subunit encodes the channel. The K conductance expressed from injection of size-fractionated mRNA was identical in all respects to that seen using unfractionated mRNA, including response to 4 beta-phorbol 12-myristate 13-acetate. The results suggest that protein kinase C regulates K secretion in epithelia by modulation of apical K channels.
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