ArticleGenomics, proteomics & bioinformatics2013
Mass spectrometry-based quantitative proteomic profiling of human pancreatic and hepatic stellate cell lines.
Article in Genomics, proteomics & bioinformatics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Mechanistic insights into post-translational modifications in hepatic fibrosis: pathogenic roles and therapeutic potentials.Journal of translational medicine · 2025Review
- Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines.International journal of molecular sciences · 2022Article
- Targeting pancreatic stellate cells in chronic pancreatitis: Focus on therapeutic drugs and natural compounds.Frontiers in pharmacology · 2022Review
- Biological and Proteomic Characteristics of an Immortalized Human Pancreatic Stellate Cell Line.International journal of medical sciences · 2020Article
- The Role of Stellate Cells in Pancreatic Ductal Adenocarcinoma: Targeting Perspectives.Frontiers in oncology · 2020Review
- Article
- AAV9-Molecular therapy. Methods & clinical development · 2019Article
- Retinoids in Stellate Cells: Development, Repair, and Regeneration.Journal of developmental biology · 2019Review
- Pancreatic Stellate Cells: A Rising Translational Physiology Star as a Potential Stem Cell Type for Beta Cell Neogenesis.Frontiers in physiology · 2019Review
- Effect of octreotide on pancreatic fibrosis in rats with high-fat diet-induced obesity.International journal of clinical and experimental pathology · 2018Article
- A Rising Star in Pancreatic Diseases: Pancreatic Stellate Cells.Frontiers in physiology · 2018Review
- Inhibition of Jak/STAT signaling reduces the activation of pancreatic stellate cells in vitro and limits caerulein-induced chronic pancreatitis in vivo.Scientific reports · 2017Article
- Pancreatic stellate cell: Pandora's box for pancreatic disease biology.World journal of gastroenterology · 2017Review
- Comparative Analysis of TwoFrontiers in microbiology · 2016Article
- Elevated temperature alters proteomic responses of individual organisms within a biofilm community.The ISME journal · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The functions of the liver and the pancreas differ; however, chronic inflammation in both organs is associated with fibrosis. Evidence suggests that fibrosis in both organs is partially regulated by organ-specific stellate cells. We explore the proteome of human hepatic stellate cells (hHSC) and human pancreatic stellate cells (hPaSC) using mass spectrometry (MS)-based quantitative proteomics to investigate pathophysiologic mechanisms. Proteins were isolated from whole cell lysates of immortalized hHSC and hPaSC. These proteins were tryptically digested, labeled with tandem mass tags (TMT), fractionated by OFFGEL, and subjected to MS. Proteins significantly different in abundance (P<0.05) were classified via gene ontology (GO) analysis. We identified 1223 proteins and among them, 1222 proteins were quantifiable. Statistical analysis determined that 177 proteins were of higher abundance in hHSC, while 157 were of higher abundance in hPaSC. GO classification revealed that proteins of relatively higher abundance in hHSC were associated with protein production, while those of relatively higher abundance in hPaSC were involved in cell structure. Future studies using the methodologies established herein, but with further upstream fractionation and/or use of enhanced MS instrumentation will allow greater proteome coverage, achieving a comprehensive proteomic analysis of hHSC and hPaSC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.