ArticleBiophysical journal2013
Tilting and wobble of myosin V by high-speed single-molecule polarized fluorescence microscopy.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
- Mapping Optical Chirality with Single Fluorescent Molecules.Nano letters · 2026Article
- Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM).bioRxiv : the preprint server for biology · 2024Article
- Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope.Nature photonics · 2023Article
- Lipid-mediated prestin organization in outer hair cell membranes and its implications in sound amplification.Nature communications · 2022Article
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
- Quantification of the NA dependent change of shape in the image formation of a z-polarized fluorescent molecule using vectorial diffraction simulations.Microscopy research and technique · 2022Article
- No hype in hyperspace.Biophysical journal · 2021Article
- Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation.Nature communications · 2020Article
- Processivity and Velocity for Motors Stepping on Periodic Tracks.Biophysical journal · 2020Article
- Article
- Structural basis for power stroke vs. Brownian ratchet mechanisms of motor proteins.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single molecule mechanics resolves the earliest events in force generation by cardiac myosin.eLife · 2019Article
- Fundamental Limits on Measuring the Rotational Constraint of Single Molecules Using Fluorescence Microscopy.Physical review letters · 2019Article
- Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function.Applied physics letters · 2018Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- STEAM: Using the Arts to Train Well-Rounded and Creative Scientists.Journal of microbiology & biology education · 2018Article
- Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Myosin V is biomolecular motor with two actin-binding domains (heads) that take multiple steps along actin by a hand-over-hand mechanism. We used high-speed polarized total internal reflection fluorescence (polTIRF) microscopy to study the structural dynamics of single myosin V molecules that had been labeled with bifunctional rhodamine linked to one of the calmodulins along the lever arm. With the use of time-correlated single-photon counting technology, the temporal resolution of the polTIRF microscope was improved ~50-fold relative to earlier studies, and a maximum-likelihood, multitrace change-point algorithm was used to objectively determine the times when structural changes occurred. Short-lived substeps that displayed an abrupt increase in rotational mobility were detected during stepping, likely corresponding to random thermal fluctuations of the stepping head while it searched for its next actin-binding site. Thus, myosin V harnesses its fluctuating environment to extend its reach. Additional, less frequent angle changes, probably not directly associated with steps, were detected in both leading and trailing heads. The high-speed polTIRF method and change-point analysis may be applicable to single-molecule studies of other biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.