ArticleAnalytical chemistry2013
Automated capillary electrophoresis system for fast single-cell analysis.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.Analytical chemistry · 2025Article
- Triple-Barrel Ultramicroelectrodes for Multipurpose, Submilliliter Electroanalysis.Analytical chemistry · 2023Article
- "Fix and Click" for Assay of Sphingolipid Signaling in Single Primary Human Intestinal Epithelial Cells.Analytical chemistry · 2022Article
- Segmentation-based analysis of single-cell immunoblots.Electrophoresis · 2021Article
- Measuring expression heterogeneity of single-cell cytoskeletal protein complexes.Nature communications · 2021Article
- Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.Nature communications · 2021Article
- Nanoelectrochemical quantification of single-cell metabolism.Analytical and bioanalytical chemistry · 2021Review
- 3D projection electrophoresis for single-cell immunoblotting.Nature communications · 2020Article
- Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells.Analytical chemistry · 2019Article
- Exploring the Fundamental Structures of Life: Non-Targeted, Chemical Analysis of Single Cells and Subcellular Structures.Angewandte Chemie (International ed. in English) · 2019Review
- Electron microscopy snapshots of single particles from single cells.The Journal of biological chemistry · 2019Article
- Design of an automated capillary electrophoresis platform for single-cell analysis.Methods in enzymology · 2019Review
- Microfluidic-Based Single-Cell Study: Current Status and Future Perspective.Molecules (Basel, Switzerland) · 2018Review
- Design and Application of Sensors for Chemical Cytometry.ACS chemical biology · 2018Review
- Review
- Microchip-based single-cell functional proteomics for biomedical applications.Lab on a chip · 2017Review
- Subcellular western blotting of single cells.Microsystems & nanoengineering · 2017Article
- An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells.Angewandte Chemie (International ed. in English) · 2016Article
- Single Cell Chemical Cytometry of Akt Activity in Rheumatoid Arthritis and Normal Fibroblast-like Synoviocytes in Response to Tumor Necrosis Factor α.Analytical chemistry · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Capillary electrophoresis (CE) is a promising technique for single-cell analysis, but its use in biological studies has been limited by low throughput. This paper presents an automated platform employing microfabricated cell traps and a three-channel system for rapid buffer exchange for fast single-cell CE. Cells loaded with fluorescein and Oregon green were analyzed at a throughput of 3.5 cells/min with a resolution of 2.3 ± 0.6 for the fluorescein and Oregon green. Cellular protein kinase B (PKB) activity, as measured by immunofluorescence staining of phospho-PKB, was not altered, suggesting that this stress-activated kinase was not upregulated during the CE experiments and that basal cell physiology was not perturbed prior to cell lysis. The activity of sphingosine kinase (SK), which is often upregulated in cancer, was measured in leukemic cells by loading a sphingosine-fluorescein substrate into cells. Sphingosine fluorescein (SF), sphingosine-1-phosphate fluorescein (S1PF), and a third fluorescent species were identified in single cells. A single-cell throughput of 2.1 cells/min was achieved for 219 total cells. Eighty-eight percent of cells possessed upregulated SK activity, although subpopulations of cells with markedly different SK activity relative to that of the population average were readily identified. This system was capable of stable and reproducible separations of biological compounds in hundreds of adherent and nonadherent cells, enabling measurements of previously uncharacterized biological phenomena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.