ArticleMolecular plant pathology2013
Similar and distinct roles of NADPH oxidase components in the tangerine pathotype of Alternaria alternata.
Article in Molecular plant pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Hydrogen peroxide as a multifaceted regulator of the Atg4 protease and autophagy in the pathogenic fungusFrontiers in fungal biology · 2026Article
- Roles of NADPH oxidases in regulating redox homeostasis and pathogenesis of the poplar canker fungus Cytospora chrysosperma.Stress biology · 2025Article
- Small GTPase Rac promotes hyphal formation and microconidiogenesis inSmall GTPases · 2024Article
- Contribution of Autophagy to Cellular Iron Homeostasis and Stress Adaptation inInternational journal of molecular sciences · 2024Article
- Infection-specific transcriptional patterns of the maize pathogen Cochliobolus heterostrophus unravel genes involved in asexual development and virulence.Molecular plant pathology · 2024Article
- The FomYjeF Protein Influences the Sporulation and Virulence ofInternational journal of molecular sciences · 2023Article
- Pexophagy is critical for fungal development, stress response, and virulence in Alternaria alternata.Molecular plant pathology · 2022Article
- Whole genome sequencing ofFrontiers in genetics · 2022Article
- NOXA Is Important forJournal of fungi (Basel, Switzerland) · 2021Article
- The critical role ofApplied and environmental microbiology · 2021Article
- Histone Acetyltransferases and Deacetylases Are Required for Virulence, Conidiation, DNA Damage Repair, and Multiple Stresses Resistance ofFrontiers in microbiology · 2021Article
- Peroxisomes Implicated in the Biosynthesis of Siderophores and Biotin, Cell Wall Integrity, Autophagy, and Response to Hydrogen Peroxide in the Citrus Pathogenic FungusFrontiers in microbiology · 2021Article
- NADPH Oxidase Regulates the Growth and Pathogenicity ofFrontiers in plant science · 2021Article
- Proper Functions of Peroxisomes Are Vital for Pathogenesis of Citrus Brown Spot Disease Caused byJournal of fungi (Basel, Switzerland) · 2020Article
- The basal transcription factor II H subunit Tfb5 is required for stress response and pathogenicity in the tangerine pathotype of Alternaria alternata.Molecular plant pathology · 2020Article
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- Cell-Wall-Degrading Enzymes Required for Virulence in the Host Selective Toxin-Producing NecrotrophFrontiers in microbiology · 2019Article
- Uncertainty surrounding the mechanism and safety of the post-harvest fungicide fludioxonil.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2019Review
- Thioredoxin and Glutaredoxin Systems Required for Oxidative Stress Resistance, Fungicide Sensitivity, and Virulence of Alternaria alternata.Applied and environmental microbiology · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungal nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) complex, which has been implicated in the production of low-level reactive oxygen species (ROS), contains mainly NoxA, NoxB (gp91(phox) homologues) and NoxR (p67(phox) homologue). Here, we report the developmental and pathological functions of NoxB and NoxR in the tangerine pathotype of Alternaria alternata. Loss-of-function genetics revealed that all three Nox components are required for the accumulation of cellular hydrogen peroxide (H₂O₂). Alternaria alternata strains lacking NoxA, NoxB or NoxR also displayed an increased sensitivity to H₂O₂ and many ROS-generating oxidants. These phenotypes are highly similar to those previously seen for the Δyap1 mutant lacking a YAP1 transcriptional regulator and for the Δhog1 mutant lacking a HOG1 mitogen-activated protein (MAP) kinase, implicating a possible link among them. A fungal strain carrying a NoxA NoxB or NoxA NoxR double mutation was more sensitive to the test compounds than the strain mutated at a single gene, implicating a synergistic function among Nox components. The ΔnoxB mutant strain failed to produce any conidia; both ΔnoxA and ΔnoxR mutant strains showed a severe reduction in sporulation. Mutant strains carrying defective NoxB had higher chitin content than the wild-type and were insensitive to calcofluor white, Congo red and the fungicides vinclozolin and fludioxonil. Virulence assays revealed that all three Nox components are required for the elaboration of the penetration process. The inability to penetrate the citrus host, observed for Δnox mutants, could be overcome by wounding and by reacquiring a dominant Nox gene. The A. alternata NoxR did not influence the expression of NoxB, but negatively regulated NoxA. Importantly, the expression of both YAP1 and HOG1 genes, whose products are involved in resistance to ROS, was down-regulated in fungi carrying defective NoxA, NoxB or NoxR. Our results highlight the requirement of Nox in ROS resistance and provide insights into its critical role in regulating both YAP1 and HOG1 in A. alternata.
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