Evidence map›Paper›PMID 23527575›Full record

ArticleACS chemical neuroscience2013

Selective catecholamine recognition with NeuroSensor 521: a fluorescent sensor for the visualization of norepinephrine in fixed and live cells.

Kenneth S Hettie, Xin Liu, Kevin D Gillis, Timothy E Glass

Open access · greenAbstract read
In one paragraph

Article in ACS chemical neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 86 citations in OpenAlex.

  1. Article
  2. Connective tissue mast cells store and release noradrenaline.The journal of physiological sciences : JPS · 2023
    Article
  3. Real-time label-free detection of dynamic aptamer-small molecule interactions using a nanopore nucleic acid conformational sensor.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
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  17. Article
  18. Targeting biomolecules with reversible covalent chemistry.Current opinion in chemical biology · 2016
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kenneth S HettieDepartment of Chemistry, University of Missouri, Columbia, Missouri 65211, United States.
Xin Liu
Kevin D Gillis
Timothy E Glass
University of Missouri · US

Funding

Development of a prototype system for assaying exocytosis from individual cellsR43MH096650 · NIMH · EXOCYTRONICS, LLC · PI GILLIS, KEVIN D, LIU, XIN ALICE · 2011 to 2012
$667k
NIMH NIH HHS R43 MH096650
6 · The paper itself

Abstract

A method for the selective labeling and imaging of catecholamines in live and fixed secretory cells is reported. The method integrates a tailored approach using a novel fluorescence-based turn-on molecular sensor (NeuroSensor 521) that can exploit the high concentration of neurotransmitters and acidic environment within secretory vesicles for the selective recognition of norepinephrine and dopamine. The utility of the method was demonstrated by selectively labeling and imaging norepinephrine in secretory vesicles such that discrimination between norepinephrine- and epinephrine-enriched populations of chromaffin cells was observed. This method was validated in fixed cells by co-staining with an anti-PNMT antibody.

Indexed as

AnimalsBinding SitesCatecholaminesCattleCells, CulturedChromaffin CellsFluorescent DyesNorepinephrineCatecholaminesFluorescent DyesNorepinephrine

Identifiers

PMID23527575
PMCPMC3708293
OpenAlexW2073260120

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.