ArticlePloS one2013
Ex vivo neurogenesis within enteric ganglia occurs in a PTEN dependent manner.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Management and outcomes for long-segment Hirschsprung disease: A systematic review from the APSA Outcomes and Evidence Based Practice Committee.Journal of pediatric surgery · 2021Pooled it
- Stress-induced glucocorticoid signaling impairs enteric neurotrophin BDNF-TrkB pathway and drives gastrointestinal dysmotility.The Journal of biological chemistry · 2026Article
- Reduced enteric BDNF-TrkB signaling drives stress-dependent glucocorticoid-mediated GI dysmotility.bioRxiv : the preprint server for biology · 2025Article
- Phosphatase and Tensin Homolog Inhibition in Proteolipid Protein 1-Expressing Cells Stimulates Neurogenesis and Gliogenesis in the Postnatal Enteric Nervous System.Biomolecules · 2024Article
- Age-associated changes in lineage composition of the enteric nervous system regulate gut health and disease.eLife · 2023Article
- The Aging Enteric Nervous System.International journal of molecular sciences · 2023Review
- [Effects of leptin on proliferation and differentiation of hypoxic rat retinal progenitor cellsNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- Pyrroloquinoline Quinone Regulates Enteric Neurochemical Plasticity of Weaned Rats Challenged With Lipopolysaccharide.Frontiers in neuroscience · 2022Article
- 37/67-laminin receptor facilitates neural crest cell migration during enteric nervous system development.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Laminin and Environmental Cues Act in the Inhibition of the Neuronal Differentiation of Enteric GliaFrontiers in neuroscience · 2019Article
- Gut microbiota regulates maturation of the adult enteric nervous system via enteric serotonin networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells.Scientific reports · 2017Article
- Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Optimizing neurogenic potential of enteric neurospheres for treatment of neurointestinal diseases.The Journal of surgical research · 2016Article
- Article
- Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants.Stem cells international · 2016Article
- Organotypic Cultures as a Model to Study Adult Neurogenesis in CNS Disorders.Stem cells international · 2016Review
- Comparative Microarray Analysis of Proliferating and Differentiating Murine ENS Progenitor Cells.Stem cells international · 2016Article
- Migration and differentiation of transplanted enteric neural crest-derived cells in murine model of Hirschsprung's disease.Cytotechnology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
A population of multipotent stem cells capable of differentiating into neurons and glia has been isolated from adult intestine in humans and rodents. While these cells may provide a pool of stem cells for neurogenesis in the enteric nervous system (ENS), such a function has been difficult to demonstrate in vivo. An extensive study by Joseph et al. involving 108 rats and 51 mice submitted to various insults demonstrated neuronal uptake of thymidine analog BrdU in only 1 rat. Here we introduce a novel approach to study neurogenesis in the ENS using an ex vivo organotypic tissue culturing system. Culturing longitudinal muscle and myenteric plexus tissue, we show that the enteric nervous system has tremendous replicative capacity with the majority of neural crest cells demonstrating EdU uptake by 48 hours. EdU(+) cells express both neuronal and glial markers. Proliferation appears dependent on the PTEN/PI3K/Akt pathway with decreased PTEN mRNA expression and increased PTEN phosphorylation (inactivation) corresponding to increased Akt activity and proliferation. Inhibition of PTEN with bpV(phen) augments proliferation while LY294002, a PI3K inhibitor, blocks it. These data suggest that the ENS is capable of neurogenesis in a PTEN dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.