ArticlePloS one2013
Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 75 citations in OpenAlex.
- Novel single-chain fragment variable antibody targeting plasma membrane epitopes on porcine Y-chromosome-bearing sperm.Scientific reports · 2025Article
- Evaluation of sperm sexing technique using BSA column medium in Garut rams: Enhancing sperm quality, kinematics, and fertility performance for improved breeding efficiency.Open veterinary journal · 2025Article
- Proteomic and Metabolomic Profiling Reveals Alterations in Boar X and Y Sperm.Animals : an open access journal from MDPI · 2024Article
- Exploring aptamers for targeted enrichment of X sperm in bovine: unraveling selective potential.Animal biotechnology · 2024Article
- The effect of toll-like receptor 7/8 ligand in inhibiting the motility of putative X-chromosome-bearing sperm in rams.Journal of advanced veterinary and animal research · 2024Article
- An Observation of the Microstructure of Cervical Mucus in Cows during the Proestrus, Estrus, and Metestrus Stages and the Impact on Sperm Penetration Ability.Veterinary sciences · 2024Article
- Article
- Metal deposition and shape reproduction at biological temperatures on cell-level samples.Scientific reports · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- Role of nanotechnology in animal production and veterinary medicine.Tropical animal health and production · 2021Review
- The micro-RNA content of unsorted cryopreserved bovine sperm and its relation to the fertility of sperm after sex-sorting.BMC genomics · 2021Article
- High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa.Animal reproduction science · 2020Article
- Separation-encoded microparticles for single-cell western blotting.Lab on a chip · 2020Article
- Differential Sperm Motility Mediates the Sex Ratio Drive Shaping Mouse Sex Chromosome Evolution.Current biology : CB · 2019Article
- Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine.Veterinary world · 2019Article
- New Biological Insights on X and Y Chromosome-Bearing Spermatozoa.Frontiers in cell and developmental biology · 2019Review
- 3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.Scientific reports · 2018Article
- Article
- The battle of the sexes starts in the oviduct: modulation of oviductal transcriptome by X and Y-bearing spermatozoa.BMC genomics · 2014Article
- Current status and potential of morphometric sperm analysis.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm dimensions and the question of whether X and Y chromosome-bearing sperm differ in size or shape has been of great interest, especially for the development of alternative methods to sort or classify sperm cells. The aim of the present study was to evaluate possible differences in the shape and size of the sperm head between X and Y chromosome-bearing sperm by atomic force microscopy (AFM). One ejaculate per bull (n = 4) was used. Each ejaculate was separated into four fractions: non-sexed (NS), sexed for X-sperm (SX), sexed for Y-sperm (SY) and a pooling of SX and SY samples (SXY). Using AFM, 400 sperm heads per group were measured. Twenty three structural features were assessed including one-, two- and three-dimensional parameters and shape descriptors. These measurements determine the micro- to nanoscale features of X- and Y-bearing chromosomes in sperm cells. No differences were observed for any individual variables between SX and SY groups. Next, a simultaneous evaluation of all features using statistical discriminant analysis was performed to determine if it was possible to distinguish to which group belong each individual cells. This analysis clearly showed, a distinct separation of NS, SXY, SX and SY groups. The recognition of this structural possibility to distinguish between X and Y sperm cell might improve the understanding of sperm cells biology. These results indicated that the associations of several structural measurements of the sperm cell head are promising candidates for development of a new method of sperm sexing.
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