ArticlePloS one2013
Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.
- The use of bacterial bioremediation of metals in aquatic environments in the twenty-first century: a systematic review.Environmental science and pollution research international · 2017Pooled it
- Engineering fusion enzymes for enhanced catalysis and stability: strategies and biotechnological applications.Molecular biology reports · 2026Review
- Key findings from 15 years ofApplied and environmental microbiology · 2025Review
- Cloning and expression of chromate reductase from Bacillus paramycoides S48 for chromium remediation.Scientific reports · 2025Article
- Mixing of Two Microbial Consortia in the Search for Stimulating Chromium Depletion.International journal of microbiology · 2025Article
- Halotolerant Endophytic BacteriaMicroorganisms · 2024Article
- Genome analysis of Shewanella putrefaciens 4H revealing the potential mechanisms for the chromium remediation.BMC genomics · 2024Article
- Isolation, molecular identification, and genomic analysis ofFrontiers in microbiology · 2024Article
- Influencing factors and mechanism of Cr(VI) reduction by facultative anaerobicFrontiers in microbiology · 2023Article
- Identification of a Novel Chromate and Selenite Reductase FesR inFrontiers in microbiology · 2022Article
- Transcriptome analysis provides new insights into the tolerance and reduction of Lysinibacillus fusiformis 15-4 to hexavalent chromium.Applied microbiology and biotechnology · 2021Article
- Metatranscriptomic analysis reveals active microbes and genes responded to short-term Cr(VI) stress.Ecotoxicology (London, England) · 2021Article
- Modeling cometabolism of hexavalent chromium by iron reducing bacteria in tertiary substrate system.Scientific reports · 2021Article
- NemA Catalyzes Trivalent Organoarsenical Oxidation and Is Regulated by the Trivalent Organoarsenical-Selective Transcriptional Repressor NemR.Environmental science & technology · 2021Article
- NfoR: Chromate Reductase or Flavin Mononucleotide Reductase?Applied and environmental microbiology · 2020Article
- Reduction of chromium-VI by chromium-resistant Escherichia coli FACU: a prospective bacterium for bioremediation.Folia microbiologica · 2020Article
- Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota.Communications biology · 2020Article
- Chemical-Assisted Microbially Mediated Chromium (Cr) (VI) Reduction Under the Influence of Various Electron Donors, Redox Mediators, and Other Additives: An Outlook on Enhanced Cr(VI) Removal.Frontiers in microbiology · 2020Review
- Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications.Frontiers in bioengineering and biotechnology · 2020Review
- Bioengineering toward direct production of immobilized enzymes: A paradigm shift in biocatalyst design.Bioengineered · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hexavalent chromium is a serious and widespread environmental pollutant. Although many bacteria have been identified that can transform highly water-soluble and toxic Cr(VI) to insoluble and relatively non-toxic Cr(III), bacterial bioremediation of Cr(VI) pollution is limited by a number of issues, in particular chromium toxicity to the remediating cells. To address this we sought to develop an immobilized enzymatic system for Cr(VI) remediation. To identify novel Cr(VI) reductase enzymes we first screened cell extracts from an Escherichia coli library of soluble oxidoreductases derived from a range of bacteria, but found that a number of these enzymes can reduce Cr(VI) indirectly, via redox intermediates present in the crude extracts. Instead, activity assays for 15 candidate enzymes purified as His6-tagged proteins identified E. coli NemA as a highly efficient Cr(VI) reductase (k(cat)/K(M)= 1.1×10(5) M(-1) s(-1) with NADH as cofactor). Fusion of nemA to the polyhydroxyalkanoate synthase gene phaC from Ralstonia eutropha enabled high-level biosynthesis of functionalized polyhydroxyalkanoate granules displaying stable and active NemA on their surface. When these granules were combined with either Bacillus subtilis glucose dehydrogenase or Candida boidinii formate dehydrogenase as a cofactor regenerating partner, high levels of chromate transformation were observed with only low initial concentrations of expensive NADH cofactor being required, the overall reaction being powered by consumption of the cheap sacrificial substrates glucose or formic acid, respectively. This system therefore offers promise as an economic solution for ex situ Cr(VI) remediation.
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