ArticlePloS one2013
Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 101 citations in OpenAlex.
- Sensitive Methanol and Formaldehyde Detection in Enzyme Assays Shows Applicability to Diverse Sample Matrices.ACS omega · 2026Article
- A Novel Nitrogen Metabolism Pathway in StrainBiology · 2026Article
- Advances in synthetic biology for engineering methylotrophic microbial cell factories.Journal of bacteriology · 2026Review
- Autotrophic methylotrophy with no methanol dehydrogenase (MDH) in a strain of fluorescentPeerJ · 2026Article
- Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.Nature communications · 2025Article
- ADP-ribose is a competitive inhibitor of methanol dehydrogenases from Bacillus methanolicus.The Journal of biological chemistry · 2025Article
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.Journal of agricultural and food chemistry · 2025Article
- Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde.Synthetic and systems biotechnology · 2023Article
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Toward Methanol-Based Biomanufacturing: Emerging Strategies for Engineering Synthetic Methylotrophy inACS synthetic biology · 2022Review
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation.Advances in biochemical engineering/biotechnology · 2022Article
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- Beyond alcohol oxidase: the methylotrophic yeast Komagataella phaffii utilizes methanol also with its native alcohol dehydrogenase Adh2.FEMS yeast research · 2021Article
- Biosensor-Based Directed Evolution of Methanol Dehydrogenase fromInternational journal of molecular sciences · 2021Article
- Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases ofFrontiers in microbiology · 2021Article
- Physiological, genomic, and sulfur isotopic characterization of methanol metabolism by Desulfovibrio carbinolicus.PloS one · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B. methanolicus strains MGA3 (ATCC53907) and PB1 (NCIMB13113) were sequenced and found to harbor three different putative Mdh encoding genes, each belonging to the type III Fe-NAD(+)-dependent alcohol dehydrogenases. In each strain, two of these genes are encoded on the chromosome and one on a plasmid; only one chromosomal act gene encoding the previously described activator protein ACT was found. The six Mdhs and the ACT proteins were produced recombinantly in Escherichia coli, purified, and characterized. All Mdhs required NAD(+) as cosubstrate, were catalytically stimulated by ACT, exhibited a broad and different substrate specificity range and displayed both dehydrogenase and reductase activities. All Mdhs catalyzed the oxidation of methanol; however the catalytic activity for methanol was considerably lower than for most other alcohols tested, suggesting that these enzymes represent a novel class of alcohol dehydrogenases. The kinetic constants for the Mdhs were comparable when acting as pure enzymes, but together with ACT the differences were more pronounced. Quantitative PCR experiments revealed major differences with respect to transcriptional regulation of the paralogous genes. Taken together our data indicate that the repertoire of methanol oxidizing enzymes in thermotolerant bacilli is larger than expected with complex mechanisms involved in their regulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.