ArticlePloS one2013
Antisense oligodeoxynucleotide inhibition as an alternative and convenient method for gene function analysis in pollen tubes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Application of Antisense Oligonucleotides as an Alternative Approach for Gene Expression Control and Functional Studies.International journal of molecular sciences · 2025Review
- Investigating vesicle-mediated regulation of pollen tube growth through BFA inhibition and AS-ODN targeting ofHorticulture research · 2025Article
- Methyl Jasmonate (MeJA) Promotes the Self-Pollen Tube Growth ofInternational journal of molecular sciences · 2024Article
- Effective Antiviral Application of Antisense in Plants by Exploiting Accessible Sites in the Target RNA.International journal of molecular sciences · 2023Article
- Regulation of Exocyst Function in Pollen Tube Growth by Phosphorylation of Exocyst Subunit EXO70C2.Frontiers in plant science · 2020Article
- Programmed Cell Death in Stigmatic Papilla Cells Is Associated With Senescence-Induced Self-Incompatibility Breakdown in Chinese Cabbage and Radish.Frontiers in plant science · 2020Article
- Knockdown of Dinoflagellate Cellulose SynthaseFrontiers in microbiology · 2019Article
- Oligodeoxynucleotides Can Transiently Up- and Downregulate CHS Gene Expression in Flax by Changing DNA Methylation in a Sequence-Specific Manner.Frontiers in plant science · 2017Article
- Oligonucleotide treatment causes flax β-glucanase up-regulation via changes in gene-body methylation.BMC plant biology · 2014Article
- Bactericidal activities of GM flax seedcake extract on pathogenic bacteria clinical strains.BMC biotechnology · 2014Article
- Article
- Potential ofIranian journal of parasitologyArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have been few successful examples to date of its application in plants, and more specifically whether the technique can be used in pollen tubes as a model of plant cell growth. NtGNL1 plays an important role in pollen tube development and was thus selected as an indicator to assess the biological effects of A-ODN. An A-ODN inhibition technique was used to down-regulate NtGNL1 expression in tobacco pollen tubes and showed that A-ODNs could quickly enter pollen tubes through the thick wall and cell membrane and effectively block NtGNL1 expression. Phenotype analysis revealed that the down-regulation of NtGNL1 by A-ODNs resulted in abnormalities in endocytosis and subsequent vesicle trafficking, similar to the phenotypes of pollen tubes treated with NtGNL1 RNAi. This investigation confirmed that A-ODNs could specifically inhibit target gene expression, and furthermore demonstrated that A-ODN functioned in a concentration- and duration-dependent manner, because A-ODNs could be degraded when incubated with pollen tubes. Thus, the A-ODN technique was successfully used for gene function analysis in pollen tubes and appears to be an alternative and convenient technique when the in vitro pollen tube is used as the study model. This technique will greatly facilitate investigations on the molecular mechanism(s) underlying pollen tube growth.
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