ArticlePloS one2013
Hypermethylation of genes detected in urine from Ghanaian adults with bladder pathology associated with Schistosoma haematobium infection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 3 of them syntheses that pooled it.
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Who cites it
26 citing papers in PubMed, 3 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Epigenetic Changes Induced by Carcinogenic Agents or Occupational Exposure with Sufficient Evidence for Bladder Cancer.Cancer prevention research (Philadelphia, Pa.) · 2025Pooled it
- Effects of Schistosoma haematobium infection and treatment on the systemic and mucosal immune phenotype, gene expression and microbiome: A systematic review.PLoS neglected tropical diseases · 2024Pooled it
- Is Toxoplasma Gondii Infection a Risk Factor for Leukemia? An Evidence-Based Meta-Analysis.Medical science monitor : international medical journal of experimental and clinical research · 2016Pooled it
- Exploring TSPAN4 promoter methylation as a diagnostic biomarker for tuberculosis.Frontiers in genetics · 2024Article
- Article
- The trend of schistosomiasis related bladder cancer in the lake zone, Tanzania: a retrospective review over 10 years period.Infectious agents and cancer · 2023Review
- The role of helminths in the development of non-communicable diseases.Frontiers in immunology · 2022Review
- Epigenetic Markers Associated with Schistosomiasis.Helminthologia · 2021Article
- Urinary schistosomiasis and the associated bladder cancer: update.Journal of the Egyptian National Cancer Institute · 2020Review
- A Review of Cancer Genetics and Genomics Studies in Africa.Frontiers in oncology · 2020Review
- Biomarker discovery and beyond for diagnosis of bladder diseases.Bladder (San Francisco, Calif.) · 2020Article
- Schistosomiasis and Bladder Cancer in Egypt: Truths and Myths.Open access Macedonian journal of medical sciences · 2019Article
- Understanding Urogenital Schistosomiasis-Related Bladder Cancer: An Update.Frontiers in medicine · 2018Review
- HaltingInternational journal of cancer management · 2017Article
- Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- RASSF1A Site-Specific Methylation Hotspots in Cancer and Correlation with RASSF1C and MOAP-1.Cancers · 2016Article
- Epigenetics in Schistosomes: What We Know and What We Need Know.Frontiers in cellular and infection microbiology · 2016Review
- Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa.International journal of endocrinology · 2016Article
- Why does infection with some helminths cause cancer?Trends in cancer · 2015Article
- Alterations in DNA methylation may be the key to early detection and treatment of schistosomal bladder cancer.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
purposeSchistosoma haematobium is associated with chronic bladder damage and may subsequently induce bladder cancer in humans, thus posing a serious threat where the parasite is endemic. Here we evaluated aberrant promoter DNA methylation as a potential biomarker to detect severe bladder damage that is associated with schistosomiasis by analyzing urine specimens. MATERIALS AND
methodsA quantitative methylation-specific PCR (QMSP) assay was used to examine the methylation status of seven genes (RASSF1A, RARβ2, RUNX3, TIMP3, MGMT, P16, ARF) in 57 urine samples obtained from volunteers that include infected and uninfected by S. haematobium from an endemic region. The Fishers Exact Test and Logistic Regression analysis were used to evaluate the methylation status with bladder damage (as assessed by ultrasound examination) in subjects with S. haematobium infection.
resultsRASSF1A and TIMP3 were significant to predict severe bladder damage both in univariate (p = 0.015 and 0.023 respectively) and in multivariate (p = 0.022 and 0.032 respectively) logistic regression analysis. Area under the receiver operator characteristic curves (AUC-ROC) for RASSF1A and TIMP3 to predict severe bladder damage were 67.84% and 63.73% respectively. The combined model, which used both RASSF1A and TIMP3 promoter methylation, resulted in significant increase in AUC-ROC compared to that of TIMP3 (77.55% vs. 63.73%.29; p = 0.023).
conclusionsIn this pilot study, we showed that aberrant promoter methylation of RASSF1A and TIMP3 are present in urine sediments of patients with severe bladder damage associated with S. haematobium infection and that may be used to develop non-invasive biomarker of S. haematobium exposure and early molecular risk assessmentof neoplastic transformation.
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