ArticlePloS one2013
Crystal structure of 70S ribosome with both cognate tRNAs in the E and P sites representing an authentic elongation complex.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Exploration of the Fusidic Acid Structure Activity Space for Antibiotic Activity.Molecules (Basel, Switzerland) · 2025Article
- Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane.Scientific reports · 2024Article
- Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.Nucleic acids research · 2023Article
- Hyper-swivel head domain motions are required for complete mRNA-tRNA translocation and ribosome resetting.Nucleic acids research · 2022Article
- Article
- The GTPase BipA expressed at low temperature inThe Journal of biological chemistry · 2018Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Recurring RNA structural motifs underlie the mechanics of L1 stalk movement.Nature communications · 2017Article
- Similarity and diversity of translational GTPase factors EF-G, EF4, and BipA: From structure to function.RNA biology · 2016Review
- Structure of the GTP Form of Elongation Factor 4 (EF4) Bound to the Ribosome.The Journal of biological chemistry · 2016Article
- Model of the pathway of -1 frameshifting: Kinetics.Biochemistry and biophysics reports · 2016Article
- Structure of BipA in GTP form bound to the ratcheted ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Model of ribosomal translocation coupled with intra- and inter-subunit rotations.Biochemistry and biophysics reports · 2015Article
- Structure of the human 80S ribosome.Nature · 2015Article
- Studying the properties of domain I of the ribosomal protein l1: incorporation into ribosome and regulation of the l1 operon expression.The protein journal · 2015Article
- Stabilization of eukaryotic ribosomal termination complexes by deacylated tRNA.Nucleic acids research · 2015Article
- The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis.RNA biology · 2015Article
- EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex.Nature structural & molecular biology · 2014Article
- Reduced ribosomes of the apicoplast and mitochondrion of Plasmodium spp. and predicted interactions with antibiotics.Open biology · 2014Article
- EF-G and EF4: translocation and back-translocation on the bacterial ribosome.Nature reviews. Microbiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
During the translation cycle, a cognate deacylated tRNA can only move together with the codon into the E site. We here present the first structure of a cognate tRNA bound to the ribosomal E site resulting from translocation by EF-G, in which an entire L1 stalk (L1 protein and L1 rRNA) interacts with E-site tRNA (E-tRNA), representing an authentic ribosome elongation complex. Our results revealed that the Watson-Crick base pairing is formed at the first and second codon-anticodon positions in the E site in the ribosome elongation complex, whereas the codon-anticodon interaction in the third position is indirect. Analysis of the observed conformations of mRNA and E-tRNA suggests that the ribosome intrinsically has the potential to form codon-anticodon interaction in the E site, independently of the mRNA configuration. We also present a detailed description of the biologically relevant position of the entire L1 stalk and its interacting cognate E-tRNA, which provides a better understanding of the structural basis for translation elongation. Furthermore, to gain insight into translocation, we report the positioning of protein L6 contacting EF-G, as well as the conformational change of the C-terminal tail of protein S13 in the decoding center.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.