ArticlePloS one2013
Knockdown of myostatin expression by RNAi enhances muscle growth in transgenic sheep.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
- A review of transgenic animal techniques and their applications.Journal, genetic engineering & biotechnology · 2023Review
- Whole Transcriptome Analysis of Differentially Expressed Genes in Cultured Nile Tilapia (Genes · 2023Article
- A Potential Negative Regulatory Function of Myostatin in the Growth of the Pacific Abalone,Biology · 2022Article
- Embryonic transcriptome unravels mechanisms and pathways underlying embryonic development with respect to muscle growth, egg production, and plumage formation in native and broiler chickens.Frontiers in genetics · 2022Article
- A Novel Nanobody Directed against Ovine Myostatin to Enhance Muscle Growth in Mouse.Animals : an open access journal from MDPI · 2020Article
- Post-transcriptional silencing of myostatin-1 in the spotted rose snapper (Lutjanus guttatus) promotes muscle hypertrophy.Molecular biology reports · 2020Article
- Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era.Frontiers in genetics · 2019Review
- Expression profiles of circular RNAs in sheep skeletal muscle.Asian-Australasian journal of animal sciences · 2018Article
- Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer.Scientific reports · 2016Article
- Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep.Scientific reports · 2016Article
- Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases.Asian-Australasian journal of animal sciences · 2016Article
- Identification of Deleterious Mutations in Myostatin Gene of Rohu Carp (Labeo rohita) Using Modeling and Molecular Dynamic Simulation Approaches.BioMed research international · 2016Article
- Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts.In vitro cellular & developmental biology. Animal · 2014Article
- Article
- Efficient gene knockout in goats using CRISPR/Cas9 system.PloS one · 2014Article
- Deep intronic mutation and pseudo exon activation as a novel muscular hypertrophy modifier in cattle.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myostatin (MSTN) has been shown to be a negative regulator of skeletal muscle development and growth. MSTN dysfunction therefore offers a strategy for promoting animal growth performance in livestock production. In this study, we investigated the possibility of using RNAi-based technology to generate transgenic sheep with a double-muscle phenotype. A shRNA expression cassette targeting sheep MSTN was used to generate stable shRNA-expressing fibroblast clones. Transgenic sheep were further produced by somatic cell nuclear transfer (SCNT) technology. Five lambs developed to term and three live lambs were obtained. Integration of shRNA expression cassette in three live lambs was confirmed by PCR. RNase protection assay showed that the shRNAs targeting MSTN were expressed in muscle tissues of three transgenic sheep. MSTN expression was significantly inhibited in muscle tissues of transgenic sheep when compared with control sheep. Moreover, transgenic sheep showed a tendency to faster increase in body weight than control sheep. Histological analysis showed that myofiber diameter of transgenic sheep M17 were bigger than that of control sheep. Our findings demonstrate a promising approach to promoting muscle growth in livestock production.
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