ArticlePloS one2013
Genomics and physiology of a marine flavobacterium encoding a proteorhodopsin and a xanthorhodopsin-like protein.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Complete genome of marine flavobacteriumMicrobiology resource announcements · 2026Article
- Effect of Light Regime onJournal of microbiology and biotechnology · 2024Article
- Omega Rhodopsins: A Versatile Class of Microbial Rhodopsins.Journal of microbiology and biotechnology · 2020Review
- Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria.Frontiers in microbiology · 2019Article
- Genome Sequence of the Proteorhodopsin-Containing Bacterium Flavobacterium sp. Strain TH167, Isolated from Cyanobacterial Aggregates in a Eutrophic Lake.Genome announcements · 2018Article
- Trajectories and Drivers of Genome Evolution in Surface-Associated Marine Phaeobacter.Genome biology and evolution · 2017Article
- Draft Genome Sequence of Flavobacterium succinicans Strain DD5b.Genome announcements · 2017Article
- Geographic Impact on Genomic Divergence as Revealed by Comparison of Nine Citromicrobial Genomes.Applied and environmental microbiology · 2016Article
- Marine Bacterial and Archaeal Ion-Pumping Rhodopsins: Genetic Diversity, Physiology, and Ecology.Microbiology and molecular biology reviews : MMBR · 2016Review
- Proteorhodopsin light-enhanced growth linked to vitamin-B1 acquisition in marine Flavobacteria.The ISME journal · 2016Article
- Diversity and functional analysis of light-driven pumping rhodopsins in marine Flavobacteria.MicrobiologyOpen · 2016Article
- Complete Genome Sequence of the Proteorhodopsin-Containing Marine Bacterium Sediminicola sp. YIK13.Genome announcements · 2016Article
- Characterization of an Unconventional Rhodopsin from the Freshwater Actinobacterium Rhodoluna lacicola.Journal of bacteriology · 2015Article
- Winter diversity and expression of proteorhodopsin genes in a polar ocean.The ISME journal · 2015Article
- Cation-Specific Conformations in a Dual-Function Ion-Pumping Microbial Rhodopsin.Biochemistry · 2015Article
- Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin: Metabolic Tools for Enhanced Algal Fitness in the Prominent Order Prymnesiales (Haptophyceae).PLoS genetics · 2015Article
- Oxidative stress and starvation in Dinoroseobacter shibae: the role of extrachromosomal elements.Frontiers in microbiology · 2015Article
- Light-driven increase in carbon yield is linked to maintenance in the proteorhodopsin-containing Photobacterium angustum S14.Frontiers in microbiology · 2015Article
- Comparative single-cell genomics reveals potential ecological niches for the freshwater acI Actinobacteria lineage.The ISME journal · 2014Article
- Complete Genome Sequence of Winogradskyella sp. Strain PG-2, a Proteorhodopsin-Containing Marine Flavobacterium.Genome announcements · 2014Article
Corrections and comments
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Authors and funding
16 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteorhodopsin (PR) photoheterotrophy in the marine flavobacterium Dokdonia sp. PRO95 has previously been investigated, showing no growth stimulation in the light at intermediate carbon concentrations. Here we report the genome sequence of strain PRO95 and compare it to two other PR encoding Dokdonia genomes: that of strain 4H-3-7-5 which shows the most similar genome, and that of strain MED134 which grows better in the light under oligotrophic conditions. Our genome analysis revealed that the PRO95 genome as well as the 4H-3-7-5 genome encode a protein related to xanthorhodopsins. The genomic environment and phylogenetic distribution of this gene suggest that it may have frequently been recruited by lateral gene transfer. Expression analyses by RT-PCR and direct mRNA-sequencing showed that both rhodopsins and the complete β-carotene pathway necessary for retinal production are transcribed in PRO95. Proton translocation measurements showed enhanced proton pump activity in response to light, supporting that one or both rhodopsins are functional. Genomic information and carbon source respiration data were used to develop a defined cultivation medium for PRO95, but reproducible growth always required small amounts of yeast extract. Although PRO95 contains and expresses two rhodopsin genes, light did not stimulate its growth as determined by cell numbers in a nutrient poor seawater medium that mimics its natural environment, confirming previous experiments at intermediate carbon concentrations. Starvation or stress conditions might be needed to observe the physiological effect of light induced energy acquisition.
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