ArticleExtremophiles : life under extreme conditions2013
Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability.
Article in Extremophiles : life under extreme conditions, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Archaeal family B DNA polymerase facilitates lagging strand DNA replication in the Thermococcales.Nucleic acids research · 2026Article
- Extended Archaeal Histone-Based Chromatin Structure Regulates Global Gene Expression inFrontiers in microbiology · 2021Article
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
- Genome Replication inFrontiers in microbiology · 2017Article
- PCNA-binding proteins in the archaea: novel functionality beyond the conserved core.Current genetics · 2016Review
- A small protein inhibits proliferating cell nuclear antigen by breaking the DNA clamp.Nucleic acids research · 2016Article
- Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer.Scientific reports · 2016Article
- PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales.Scientific reports · 2016Article
- A novel mechanism for regulating the activity of proliferating cell nuclear antigen by a small protein.Nucleic acids research · 2014Article
- Global phylogenomic analysis disentangles the complex evolutionary history of DNA replication in archaea.Genome biology and evolution · 2014Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. Most eukarya and euryarchaea have only one PCNA homolog but Thermococcus kodakarensis uniquely has two, designated PCNA1 and PCNA2, encoded by TK0535 and TK0582, respectively. Here, we establish that both PCNA1 and PCNA2 form homotrimers that stimulate DNA synthesis by archaeal DNA polymerases B and D and ATP hydrolysis by the replication factor C complex. In exponentially growing cells, PCNA1 is abundant and present at an ~100-fold higher concentration than PCNA2 monomers. Deletion of TK0582 (PCNA2) had no detectable effects on viability or growth whereas repeated attempts to construct a T. kodakarensis strain with TK0535 (PCNA1) deleted were unsuccessful. The implications of these observations for PCNA1 function and the origin of the two PCNA-encoding genes in T. kodakarensis are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.