ArticleNutrients2013
Total vitamin D assay comparison of the Roche Diagnostics "Vitamin D total" electrochemiluminescence protein binding assay with the Chromsystems HPLC method in a population with both D2 and D3 forms of vitamin D.
Article in Nutrients, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 72 citations in OpenAlex.
- High-dose versus low-dose ergocalciferol for correcting hypovitaminosis D after fragility hip fracture: a randomized controlled trial.BMC geriatrics · 2021Trial
- Association of nutrient intake and dietary patterns with serum folate and anemia-related biomarkers in Taiwanese pregnant women with pre-pregnancy overweightness or obesity.International journal of medical sciences · 2025Article
- Article
- Vitamin D Deficiency Does Not Affect Cognition and Neurogenesis in Adult C57Bl/6 Mice.Nutrients · 2024Article
- Unraveling the complex interplay between obesity and vitamin D metabolism.Scientific reports · 2024Article
- Vitamin D Status, VDR, and TLR Polymorphisms and Pulmonary Tuberculosis Epidemiology in Kazakhstan.Nutrients · 2024Article
- Seasonal Variations in 25-Hydroxyvitamin D Levels among Pediatric Patients Attending the Healthcare Centre.Nutrients · 2024Article
- Associations of Dietary Patterns and Vitamin D Levels with Iron Status in Pregnant Women: A Cross-Sectional Study in Taiwan.Nutrients · 2023Article
- A Prospective Analysis of Vitamin D Levels in Pregnant Women Diagnosed with Gestational Hypertension after SARS-CoV-2 Infection.Journal of personalized medicine · 2023Article
- Article
- Article
- Association of Vitamins D, BFood technology and biotechnology · 2022Article
- Vitamin D: a 14-year retrospective study at a clinical laboratory in Brazil.Archives of endocrinology and metabolism · 2022Article
- Vitamin D Level in Patients with Consecutive Acute Coronary Syndrome Is Not Correlated with the Parameters of Platelet Activity.Journal of clinical medicine · 2022Article
- Cystatin C, Vitamin D and Thyroid Function Test Profile in Chronic Kidney Disease Patients.Diseases (Basel, Switzerland) · 2021Article
- Article
- Vitamin D receptor genetic polymorphisms are associated with oral lichen planus susceptibility in a Chinese Han population.BMC oral health · 2020Article
- Aqueous humor and serum 25-Hydroxyvitamin D levels in patients with cataracts.BMC ophthalmology · 2020Article
- Vitamin D level is associated with severity of coronary artery atherosclerosis and incidence of acute coronary syndromes in non-diabetic cardiac patients.Archives of medical science : AMS · 2019Article
- Higher Vitamin D Levels Are Associated with Better Attentional Functions: Data from the NorCog Register.The journal of nutrition, health & aging · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study compared two methods of assaying the 25-hydroxylated metabolites of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2). A fully automated electrochemiluminescence assay from Roche Diagnostics and an HPLC based method from Chromsystems were used to measure vitamin D levels in surplus sera from 96 individuals, where the majority has the D2 form of the vitamin. Deming regression, concordance rate, correlation and Altman Bland agreement were performed. Seventy two subjects (75%) had a D2 concentration >10 nmol/L while the remaining twenty four subjects had vitamin D2 concentration of less than 10 nmol/L by HPLC. Overall, the Roche Diagnostics method showed a negative bias of -2.59 ± 4.11 nmol/L on the e602 as compared to the HPLC with a concordance rate of 84%. The concordance rate was 91% in samples with D2 of less than 10 nmol/L and 82% in those with D2 concentration >10 nmol/L. The overall correlation had an r value of 0.77. The r value was higher in samples with D2 levels of less than 10 nmol/L, r = 0.96, as compared to those with D2 values of greater than 10 nmol/L, r = 0.74. The observed bias had little impact on clinical decision and therefore is clinically acceptable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.