ArticleJournal of visualized experiments : JoVE2013
Isolating and immunostaining lymphocytes and dendritic cells from murine Peyer's patches.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Sex-dependent protective responses of taurine in ameliorating radiation-induced intestinal injury: gut microbiota reprogramming in males and estrogen-dependent immunity in females.International journal of biological sciences · 2026Article
- Issues for patchy tissues: defining roles for gut-associated lymphoid tissue in neurodevelopment and disease.Journal of neural transmission (Vienna, Austria : 1996) · 2023Review
- Label-free cell segmentation of diverse lymphoid tissues in 2D and 3D.Cell reports methods · 2023Article
- Utility of CD138/syndecan-1 immunohistochemistry for localization of plasmacytes is tissue-dependent in B6 mice.BMC research notes · 2022Article
- Effects of Koumiss on Intestinal Immune Modulation in Immunosuppressed Rats.Frontiers in nutrition · 2022Article
- Intestinal Immune System and Amplification of Mouse Mammary Tumor Virus.Frontiers in cellular and infection microbiology · 2021Review
- "Western Diet"-Induced Adipose Inflammation Requires a Complex Gut Microbiota.Cellular and molecular gastroenterology and hepatology · 2020Article
- Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches.Journal of visualized experiments : JoVE · 2018Article
- Atrophy of skin-draining lymph nodes predisposes for impaired immune responses to secondary infection in mice with chronic intestinal nematode infection.PLoS pathogens · 2018Article
- RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technology.Journal of biological methods · 2018Article
- Rapid effects of a protective O-polysaccharide-specific monoclonal IgA on Vibrio cholerae agglutination, motility, and surface morphology.Infection and immunity · 2015Article
- Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches.PloS one · 2015Article
- Role of Maternal Dietary Peanut Exposure in Development of Food Allergy and Oral Tolerance.PloS one · 2015Article
- Oral interleukin-10 alleviates polyposis via neutralization of pathogenic T-regulatory cells.Cancer research · 2014Article
- Mice deficient in intestinal epithelium cytochrome P450 reductase are prone to acute toxin-induced mucosal damage.Scientific reports · 2014Article
- A population of Langerin-positive dendritic cells in murine Peyer's patches involved in sampling β-glucan microparticles.PloS one · 2014Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Peyer's patches (PPs) are integral components of the gut-associated lymphoid tissues (GALT) and play a central role in intestinal immunosurveillance and homeostasis. Particulate antigens and microbes in the intestinal lumen are continuously sampled by PP M cells in the follicle-associated epithelium (FAE) and transported to an underlying network of dendritic cells (DCs), macrophages, and lymphocytes. In this article, we describe protocols in which murine PPs are (i) dissociated into single cell suspensions and subjected to flow cytometry and (ii) prepared for cryosectioning and immunostaining. For flow cytometry, PPs are mechanically dissociated and then filtered through 70 μm membranes to generate single cell suspensions free of epithelial cells and large debris. Starting with 20-25 PPs (from four mice), this quick and reproducible method yields a population of >2.5 x 10(6) cells with >90% cell viability. For cryosectioning, freshly isolated PPs are immersed in Optimal Cutting Temperature (OCT) medium, snap-frozen in liquid nitrogen, and then sectioned using a cryomicrotome. Tissue sections (5-12 μm) are air-dried, fixed with acetone or methanol, and then subjected to immunolabeling.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.