ArticleThe EMBO journal2013
The splicing machinery promotes RNA-directed DNA methylation and transcriptional silencing in Arabidopsis.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- The pre-mRNA splicing factor ZOP1 contributes to the regulation of plant immunity.Plant physiology · 2025Article
- PhpCNature communications · 2025Article
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- WRKY transcription factors and OBERON histone-binding proteins form complexes to balance plant growth and stress tolerance.The EMBO journal · 2023Article
- Analysis of the Arabidopsis coilin mutant reveals a positive role of AtCOILIN in plant immunity.Plant physiology · 2022Article
- A genetic screen in Arabidopsis reveals the identical roles for RBP45d and PRP39a in 5' cryptic splice site selection.Frontiers in plant science · 2022Article
- Dual Recognition of H3K4me3 and DNA by the ISWI Component ARID5 Regulates the Floral Transition in Arabidopsis.The Plant cell · 2020Article
- To Splice or to Transcribe: SKIP-Mediated Environmental Fitness and Development in Plants.Frontiers in plant science · 2019Review
- Dynamics and function of DNA methylation in plants.Nature reviews. Molecular cell biology · 2018Review
- Hypermethylation of protocadherin γ subfamily A12 and solute carrier family 19 A 1 promoters contributes to the occurrence and metastasis of colorectal cancer.Oncology letters · 2018Article
- The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing.The Plant cell · 2017Article
- Alternative Splicing in Plant Genes: A Means of Regulating the Environmental Fitness of Plants.International journal of molecular sciences · 2017Review
- The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing.Nature communications · 2016Article
- Myriad Triple-Helix-Forming Structures in the Transposable Element RNAs of Plants and Fungi.Cell reports · 2016Article
- Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors.Plant physiology · 2016Article
- RNA-directed DNA methylation in plants.Plant cell reports · 2015Article
- NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis.The EMBO journal · 2015Article
- siRNA-directed DNA Methylation in Plants.Current genomics · 2015Article
- Detection of Pol IV/RDR2-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis.Genome research · 2015Article
- Stress-induced endogenous siRNAs targeting regulatory intron sequences in Brachypodium.RNA (New York, N.Y.) · 2015Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation in transposons and other DNA repeats is conserved in plants as well as in animals. In Arabidopsis thaliana, an RNA-directed DNA methylation (RdDM) pathway directs de novo DNA methylation. We performed a forward genetic screen for suppressors of the DNA demethylase mutant ros1 and identified a novel Zinc-finger and OCRE domain-containing Protein 1 (ZOP1) that promotes Pol IV-dependent siRNA accumulation, DNA methylation, and transcriptional silencing. Whole-genome methods disclosed the genome-wide effects of zop1 on Pol IV-dependent siRNA accumulation and DNA methylation, suggesting that ZOP1 has both RdDM-dependent and -independent roles in transcriptional silencing. We demonstrated that ZOP1 is a pre-mRNA splicing factor that associates with several typical components of the splicing machinery as well as with Pol II. Immunofluorescence assay revealed that ZOP1 overlaps with Cajal body and is partially colocalized with NRPE1 and DRM2. Moreover, we found that the other development-defective splicing mutants tested including mac3a3b, mos4, mos12 and mos14 show defects in RdDM and transcriptional silencing. We propose that the splicing machinery rather than specific splicing factors is involved in promoting RdDM and transcriptional silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.