ArticleJournal of visualized experiments : JoVE2013
Transplantation into the anterior chamber of the eye for longitudinal, non-invasive in vivo imaging with single-cell resolution in real-time.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 30 citations in OpenAlex.
- Small molecules promote the in vitro expansion and passaging of adult mouse pancreatic islets.Biochemistry and biophysics reports · 2026Article
- Local and Systemic Immunologic Profiles Differentiate Accepted and Rejected Islet Grafts in a Rat Anterior Chamber Model.Journal of immunology research · 2026Article
- Investigating Late-Stage Diabetic Retinopathy: A Long-term Analysis of Vascular Changes in the Streptozotocin-Induced Mouse Model.Diabetes · 2025Article
- β-Cell Neogenesis From the Pancreatic Ductal Epithelium Revealed Dynamically by Long-term Intravital Imaging.Diabetes · 2025Article
- The IsletTester Mouse: An Immunodeficient Model With Stable Hyperglycemia for the Study of Human Islets.Diabetes · 2025Article
- The anterior chamber of the eye technology and its anatomical, optical, and immunological bases.Physiological reviews · 2024Review
- Small-molecule inhibitors of the CD40-CD40L costimulatory interaction are effective in pancreatic islet transplantation and prevention of type 1 diabetes models.Frontiers in immunology · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Mouse models and human islet transplantation sites for intravital imaging.Frontiers in endocrinology · 2022Review
- HGAL inhibits lymphoma dissemination by interacting with multiple cytoskeletal proteins.Blood advances · 2021Article
- The Human Islet: Mini-Organ With Mega-Impact.Endocrine reviews · 2021Review
- Integrated Metabolomics and Proteomics Analyses in the Local Milieu of Islet Allografts in Rejection versus Tolerance.International journal of molecular sciences · 2021Article
- Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose.Scientific reports · 2021Article
- Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse.eLife · 2020Article
- Longitudinal proteomics analysis in the immediate microenvironment of islet allografts during progression of rejection.Journal of proteomics · 2020Article
- Investigation of Cytotoxic T Lymphocyte Function during Allorejection in the Anterior Chamber of the Eye.International journal of molecular sciences · 2020Article
- A machine learning approach to predict pancreatic islet grafts rejection versus tolerance.PloS one · 2020Article
- Feasibility of Localized Metabolomics in the Study of Pancreatic Islets and Diabetes.Metabolites · 2019Article
- In vivo imaging of type 1 diabetes immunopathology using eye-transplanted islets in NOD mice.Diabetologia · 2019Article
- Operational immune tolerance towards transplanted allogeneic pancreatic islets in mice and a non-human primate.Diabetologia · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Intravital imaging has emerged as an indispensable tool in biological research. In the process, many imaging techniques have been developed to study different biological processes in animals non-invasively. However, a major technical limitation in existing intravital imaging modalities is the inability to combine non-invasive, longitudinal imaging with single-cell resolution capabilities. We show here how transplantation into the anterior chamber of the eye circumvents such significant limitation offering a versatile experimental platform that enables non-invasive, longitudinal imaging with cellular resolution in vivo. We demonstrate the transplantation procedure in the mouse and provide representative results using a model with clinical relevance, namely pancreatic islet transplantation. In addition to enabling direct visualization in a variety of tissues transplanted into the anterior chamber of the eye, this approach provides a platform to screen drugs by performing long-term follow up and monitoring in target tissues. Because of its versatility, tissue/cell transplantation into the anterior chamber of the eye not only benefits transplantation therapies, it extends to other in vivo applications to study physiological and pathophysiological processes such as signal transduction and cancer or autoimmune disease development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.