Evidence map›Paper›PMID 23524108›Full record

ArticleCarbohydrate research2013

Preparation and application of a 'clickable' acceptor for enzymatic synthesis of heparin oligosaccharides.

Chao Cai, Kristi Edgar, Jian Liu, Robert J Linhardt

Abstract read
In one paragraph

Article in Carbohydrate research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Enzymatic Synthesis of Glycans and Glycoconjugates.Advances in biochemical engineering/biotechnology · 2021
    Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chao CaiDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Kristi Edgar
Jian Liu
Robert J Linhardt

Funding

In vitro synthesis of recombinant heparan sulfateR01HL094463 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LIU, JIAN, PAWLINSKI, RAFAL L · 2009 to 2021
$5.2M
Synthetic Heparan Sulfate: Probing Biosynthesis to Prepare Defined DrugsR01HL062244 · NHLBI · UNIVERSITY OF IOWA · PI DEANGELIS, PAUL L, LINHARDT, ROBERT J · 2001 to 2016
$4.1M
SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANSR01GM038060 · NIGMS · UNIVERSITY OF IOWA · PI LINHARDT, ROBERT J · 1988 to 2012
$2.8M
NHLBI NIH HHS HL094463NHLBI NIH HHS HL62244NHLBI NIH HHS R01 HL062244NHLBI NIH HHS R01 HL094463NIGMS NIH HHS GM38060NIGMS NIH HHS R01 GM038060
6 · The paper itself

Abstract

A 'clickable' disaccharide was prepared by treating the aldehyde precursor with hydroxylamine, followed by the catalytic hydrogenation and diazotransfer reaction. This disaccharide was successfully applied to the elongation of the backbone construction of ultralow molecular weight (ULMW) heparins using two bacterial glycosyl transferases, N-acetyl glucosaminyl transferase from Escherichia coli K5 (KfiA) and heparosan synthase-2 (pmHS2) from Pasteurella multocida.

Indexed as

Carbohydrate SequenceChemistry Techniques, SyntheticDisaccharidesEscherichia coli ProteinsGlycosylationGlycosyltransferasesHeparinHeparin, Low-Molecular-WeightHydrogenationHydroxylamineMolecular Sequence DataMolecular WeightN-AcetylglucosaminyltransferasesPasteurella multocidaDisaccharidesEscherichia coli ProteinsGlycosyltransferasesHeparinHeparin, Low-Molecular-Weightheparosanheparosan synthaseHydroxylamineKfiA protein, E coliN-Acetylglucosaminyltransferases

Identifiers

PMID23524108
PMCPMC3638765

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.