ArticleMethods (San Diego, Calif.)2013
Tracking unfolding and refolding reactions of single proteins using atomic force microscopy methods.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Label-Free Single Protein Dynamics Revealed by Metasurface-Enhanced Raman Spectroscopy.ACS nano · 2026Article
- Single-Molecule Techniques for Probing the Conformation, Dynamics and Interactions of Intrinsically Disordered Proteins.Biophysics reviews · 2025Article
- Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy.Nature communications · 2025Article
- Optogenetic induction of mechanical muscle stress identifies myosin regulatory ubiquitin ligase NHL-1 in C. elegans.Nature communications · 2024Article
- Piconewton Forces Mediate GAIN Domain Dissociation of the Latrophilin-3 Adhesion GPCR.Nano letters · 2023Article
- Using steered molecular dynamic tension for assessing quality of computational protein structure models.Journal of computational chemistry · 2022Article
- From dynamic self-organization to avalanching instabilities in soft-granular threads.Soft matter · 2022Article
- Monitoring the binding and insertion of a single transmembrane protein by an insertase.Nature communications · 2021Article
- Reproducibility in the unfolding process of protein induced by an external electric field.Chemical science · 2020Article
- A Region of UNC-89 (Obscurin) Lying between Two Protein Kinase Domains Is a Highly Elastic Spring Required for Proper Sarcomere Organization.Journal of molecular biology · 2020Article
- Review
- Mechanical unfolding of spectrin reveals a super-exponential dependence of unfolding rate on force.Scientific reports · 2019Article
- Imaging and Force Recognition of Single Molecular Behaviors Using Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2017Review
- AFM/TIRF force clamp measurements of neurosecretory vesicle tethers reveal characteristic unfolding steps.PloS one · 2017Article
- Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy.Acta pharmacologica Sinica · 2015Review
- Electrically facilitated translocation of protein through solid nanopore.Nanoscale research letters · 2014Article
- Nanoimaging methods for biomedicine.Methods (San Diego, Calif.) · 2013Article
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Authors and funding
2 authors.
Funding
Abstract
During the last two decades single-molecule manipulation techniques such as atomic force microscopy (AFM) has risen to prominence through their unique capacity to provide fundamental information on the structure and function of biomolecules. Here we describe the use of single-molecule AFM to track protein unfolding and refolding pathways, enzymatic catalysis and the effects of osmolytes and chaperones on protein stability and folding. We will outline the principles of operation for two different AFM pulling techniques: length clamp and force-clamp and discuss prominent applications. We provide protocols for the construction of polyproteins which are amenable for AFM experiments, the preparation of different coverslips, choice and calibration of AFM cantilevers. We also discuss the selection criteria for AFM recordings, the calibration of AFM cantilevers, protein sample preparations and analysis of the obtained data.
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