ArticleBiomaterials2013
A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Cationic microparticles inhibit local sterile inflammation in tissue injuries.Regenerative biomaterials · 2026Article
- Progress and challenges of functionalized bacterial encapsulation: A novel biotechnology for next-generation biotherapeutics.Acta pharmaceutica Sinica. B · 2025Review
- Artificial Intelligence-Empowered Automated Double Emulsion Droplet Library Generation.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics.Micromachines · 2023Article
- Article
- Systematic characterization of effect of flow rates and buffer compositions on double emulsion droplet volumes and stability.Lab on a chip · 2022Article
- Tracking the stochastic growth of bacterial populations in microfluidic droplets.Physical biology · 2022Article
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey.Angewandte Chemie (International ed. in English) · 2021Review
- Droplet flow cytometry for single-cell analysis.RSC advances · 2021Review
- Optimisation of bacterial release from a stable microfluidic-generated water-in-oil-in-water emulsion.RSC advances · 2021Article
- Bacterial Behavior in Confined Spaces.Frontiers in cell and developmental biology · 2021Review
- Dielectrophoresis Response of Water-in-Oil-in-Water Double Emulsion Droplets with Singular or Dual Cores.Micromachines · 2020Article
- FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells.Scientific reports · 2020Article
- Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery.Lab on a chip · 2020Article
- "Basicles": Microbial Growth and Production Monitoring in Giant Lipid Vesicles.ACS applied materials & interfaces · 2019Article
- Oscillating dispersed-phase co-flow microfluidic droplet generation: Multi-droplet size effect.Biomicrofluidics · 2018Article
- High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.Nanoscale · 2017Article
- Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A versatile platform for surface modification of microfluidic droplets.Lab on a chip · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single emulsion droplets are incapable of continuous supply of nutrient molecule and are not compatible with aqueous phase-based analysis. A solution is to entrap W/O droplets in another aqueous phase, forming water-in-oil-in-water (W/O/W) double emulsions. The external aqueous phase efficiently prevents desiccation and reduces the amount of organic component, and yet retaining the advantages of compartmentalization. The internal environment can also be programmed dynamically without the need of rupturing the droplets. In this study, we explore the potential application of W/O/W double emulsion droplets for cell cultivation, genetic activation and study of more complicated biological events such as bacteria quorum-sensing as an example. This study demonstrates the advantages and potential application of double emulsion for the study of complex biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.