ArticleBMC plant biology2013
A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Dual Action of Ivy and Strawberry Essential Oils: Induction ofInternational journal of molecular sciences · 2025Article
- Comparative transcriptome and metabolite survey reveal key pathways involved in the control of the chilling injury disorder superficial scald in two apple cultivars, 'Granny Smith' and 'Ladina'.Frontiers in plant science · 2023Article
- New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1.Journal of agricultural and food chemistry · 2022Article
- Identification of apple cultivars hypoallergenic for birch pollen-allergic individuals by a multidisciplinary in vitro and in vivo approach.Clinical and translational allergy · 2022Article
- Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS.Food chemistry. Molecular sciences · 2022Article
- A TIR-NBS-LRR GeneInternational journal of molecular sciences · 2022Article
- Analysis of Sequence Variability and Transcriptional Profile ofPlants (Basel, Switzerland) · 2021Article
- Apple allergy: Causes and factors influencing fruits allergenic properties-Review.Clinical and translational allergy · 2021Review
- Molecular and Immunological Identification of Low Allergenic Fruits among Old and New Apple Varieties.International journal of molecular sciences · 2021Article
- Tiered approach for the identification of Mal d 1 reduced, well tolerated apple genotypes.Scientific reports · 2020Article
- Investigation of the Effects of Apple Trees Infection byReports of biochemistry & molecular biology · 2020Article
- Specific region affects the difference in accumulation levels between apple food allergen Mal d 1 and birch pollen allergen Bet v 1 which are expressed in vegetative tissues of transgenic rice.Plant molecular biology · 2018Article
- Structure of the Major Apple Allergen Mal d 1.Journal of agricultural and food chemistry · 2017Article
- New traits in crops produced by genome editing techniques based on deletions.Plant biotechnology reports · 2017Review
- NMR resonance assignments of the major apple allergen Mal d 1.Biomolecular NMR assignments · 2016Article
- Erratum to: A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes.BMC plant biology · 2016Article
- Article
- Quantitative and qualitative differences in celiac disease epitopes among durum wheat varieties identified through deep RNA-amplicon sequencing.BMC genomics · 2013Article
- RNA-Seq analysis reveals candidate genes for ontogenic resistance in Malus-Venturia pathosystem.PloS one · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA considerable number of individuals suffer from oral allergy syndrome (OAS) to apple, resulting in the avoidance of apple consumption. Apple cultivars differ greatly in their allergenic properties, but knowledge of the causes for such differences is incomplete. Mal d 1 is considered the major apple allergen. For Mal d 1, a wide range of isoallergens and variants exist, and they are encoded by a large gene family. To identify the specific proteins/genes that are potentially involved in the allergy, we developed a PCR assay to monitor the expression of each individual Mal d 1 gene. Gene-specific primer pairs were designed for the exploitation of sequence differences among Mal d 1 genes. The specificity of these primers was validated using both in silico and in vitro techniques. Subsequently, this assay was applied to the peel and flesh of fruits from the two cultivars 'Florina' and 'Gala'.
resultsWe successfully developed gene-specific primer pairs for each of the 31 Mal d 1 genes and incorporated them into a qRT-PCR assay. The results from the application of the assay showed that 11 genes were not expressed in fruit. In addition, differential expression was observed among the Mal d 1 genes that were expressed in the fruit. Moreover, the expression levels were tissue and cultivar dependent.
conclusionThe assay developed in this study facilitated the first characterisation of the expression levels of all known Mal d 1 genes in a gene-specific manner. Using this assay on different fruit tissues and cultivars, we obtained knowledge concerning gene relevance in allergenicity. This study provides new perspectives for research on both plant breeding and immunotherapy.
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