ArticleCNS neuroscience & therapeutics2013
Glutathione prevents free fatty acids-induced oxidative stress and apoptosis in human brain vascular endothelial cells through Akt pathway.
Article in CNS neuroscience & therapeutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Liraglutide suppresses non-esterified free fatty acids and soluble vascular cell adhesion molecule-1 compared with metformin in patients with recent-onset type 2 diabetes.Cardiovascular diabetology · 2018Trial
- Alzheimer's Disease Pathogenic Mechanisms: Linking Redox Homeostasis and Mitochondria-Associated Metabolic Pathways Through Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2).Antioxidants (Basel, Switzerland) · 2025Review
- Glutathione: A Key Regulator of Extracellular Matrix and Cell Death in Intervertebral Disc Degeneration.Mediators of inflammation · 2024Review
- Quantitative proteomic analyses uncover regulatory roles of Nrf2 in human endothelial cells.Cell stress & chaperones · 2023Article
- Hypothesis: Efficacy of early treatments with some NSAIDs in COVID-19: Might it also depend on their direct and/or indirect zinc chelating ability?British journal of pharmacology · 2023Article
- Effects of Acute and Chronic Resistance Exercise on the Skeletal Muscle Metabolome.Metabolites · 2022Article
- Dynamic metabolic change of cancer cells induced by natural killer cells at the single-cell level studied by label-free mass cytometry.Chemical science · 2022Article
- Ramelteon Mitigates Free Fatty Acid (FFA)-Induced Attachment of Monocytes to Brain Vascular Endothelial Cells.Neurotoxicity research · 2021Article
- The role of serum monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) in cardiovascular disease risk.Acta bio-medica : Atenei Parmensis · 2021Article
- Salivary Biomarkers of Oxidative Stress and Inflammation in Stroke Patients: From Basic Research to Clinical Practice.Oxidative medicine and cellular longevity · 2021Review
- Agomelatine Attenuates Isoflurane-Induced Inflammation and Damage in Brain Endothelial Cells.Drug design, development and therapy · 2020Article
- UPLC-QTOF/MS-Based Metabolomics Reveals the Protective Mechanism of Hydrogen on Mice with Ischemic Stroke.Neurochemical research · 2019Article
- Insulin Resistance and Oxidative Stress in the Brain: What's New?International journal of molecular sciences · 2019Review
- Data on metabolic-dependent antioxidant response in the cardiovascular tissues of living zebrafish under stress conditions.Data in brief · 2017Article
- G-CSF and cognitive dysfunction in elderly diabetic mice with cerebral small vessel disease: Preventive intervention effects and underlying mechanisms.CNS neuroscience & therapeutics · 2017Article
- The Role of Nrf2 in Cardiovascular Function and Disease.Oxidative medicine and cellular longevity · 2017Review
- Research Progress on Signaling Pathway-Associated Oxidative Stress in Endothelial Cells.Oxidative medicine and cellular longevity · 2017Review
- Beclin-1- mediated autophagy may be involved in the elderly cognitive and affective disorders in streptozotocin-induced diabetic mice.Translational neurodegeneration · 2016Article
- Angiopoietin-like 4: A double-edged sword in atherosclerosis and ischemic stroke?Experimental neurology · 2015Article
- ROS signaling and redox biology in endothelial cells.Cellular and molecular life sciences : CMLS · 2015Review
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe damage of human brain vascular endothelial cells (HBVECs) is the key pathogenesis of diabetes-associated cerebral vascular complications. The aim of this study was to elucidate the effects of glutathione (GSH) on free fatty acids (FFAs)-induced HBVECs apoptosis, oxidative stress, and the involved possible signaling pathway.
methodsAfter culturing HBVECs for 72 h with GSH and FFAs, we determined cell proliferation by CCK8, detected apoptosis by caspase-3 and Annexin V-FITC/PI staining, and judged oxygen stress by determining the reactive oxygen species (ROS) and the mitochondrial membrane potential (MMP). We investigated whether the Akt pathway was involved in FFAs-induced signaling pathway alteration and whether GSH influenced the above effects.
resultsAfter being cultured in 200 μM FFAs for 72 h, the HBVECs proliferation significantly decreased; HBVECs apoptosis increased; the ROS levels increased; and the HBVECs MMP subsequently decreased. FFAs induced a significant decrease in phosphorylated active Akt. These alterations were obviously prevented when 1 mM GSH was added to culture medium containing FFAs, and the above effects of GSH were blocked by Akt inhibitor.
conclusionGSH may prevent FFAs-induced HBVECs damage, oxidative stress, and apoptosis through activating the Akt pathway.
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