ArticleInsect molecular biology2013
Amblyomma americanum tick saliva serine protease inhibitor 6 is a cross-class inhibitor of serine proteases and papain-like cysteine proteases that delays plasma clotting and inhibits platelet aggregation.
Article in Insect molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 64 citations in OpenAlex.
- Chromosome-Scale Atlas ofGenes · 2026Article
- Tick-Borne Encephalitis in the 21Le infezioni in medicina · 2026Review
- Serpins in the Spotlight: Novel Bioinformatic Insights IntoBiochemistry research international · 2025Article
- GW4869 inhibitor affects vector competence and tick-borne flavivirus acquisition and transmission by blocking exosome secretion.iScience · 2024Article
- Article
- Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions.Frontiers in cellular and infection microbiology · 2022Review
- Transcriptomic analysis of the tick midgut and salivary gland responses upon repeated blood-feeding on a vertebrate host.Frontiers in cellular and infection microbiology · 2022Article
- Serpins in Tick Physiology and Tick-Host Interaction.Frontiers in cellular and infection microbiology · 2022Review
- Potential Mechanisms of Transmission of Tick-Borne Viruses at the Virus-Tick Interface.Frontiers in microbiology · 2022Review
- Tick host immunity: vector immunomodulation and acquired tick resistance.Trends in immunology · 2021Review
- A Comprehensive Phylogenetic Analysis of the Serpin Superfamily.Molecular biology and evolution · 2021Article
- Insights into the Role of Tick Salivary Protease Inhibitors during Ectoparasite-Host Crosstalk.International journal of molecular sciences · 2021Review
- De novo assembled salivary gland transcriptome and expression pattern analyses for Rhipicephalus evertsi evertsi Neuman, 1897 male and female ticks.Scientific reports · 2021Article
- Amblyomma americanum serpin 41 (AAS41) inhibits inflammation by targeting chymase and chymotrypsin.International journal of biological macromolecules · 2020Article
- Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa.Parasites & vectors · 2020Article
- Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding.PLoS neglected tropical diseases · 2020Article
- Amblyomma americanum serpin 27 (AAS27) is a tick salivary anti-inflammatory protein secreted into the host during feeding.PLoS neglected tropical diseases · 2019Article
- Immunomodulatory effects of Rhipicephalus haemaphysaloides serpin RHS2 on host immune responses.Parasites & vectors · 2019Article
- Proteomic analysis of saliva from partially and fully engorged adult female Rhipicephalus microplus (Acari: Ixodidae).Experimental & applied acarology · 2019Article
- Brown Spider (Toxins · 2019Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that Amblyomma americanum tick serine protease inhibitor 6 (AamS6) was secreted into the host during tick feeding and that both its mRNA and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. This study demonstrates that AamS6 is a cross-class inhibitor of both serine- and papain-like cysteine proteases that has apparent antihaemostatic functions. Consistent with the typical inhibitory serpin characteristics, enzyme kinetics analyses revealed that Pichia pastoris-expressed recombinant (r) AamS6 reduced initial velocities of substrate hydrolysis (V₀) and/or maximum enzyme velocity (V(max)) of trypsin, chymotrypsin, elastase, chymase, and papain in a dose-response manner. We speculate that rAamS6 inhibited plasmin in a temporary fashion in that while rAamS6 reduced V₀ of plasmin by up to ∼53%, it had no effect on V(max). Our data also suggest that rAmS6 has minimal or no apparent effect on V₀ or V(max) of thrombin, factor Xa, and kallikrein. We speculate that AamS6 is apparently involved in facilitating blood meal feeding in that various amounts of rAamS6 reduced platelet aggregation by up to ∼47% and delayed plasma clotting time in the recalcification time assay by up to ∼210 s. AamS6 is most likely not involved with the tick's evasion of the host's complement defense mechanism, in that rAamS6 did not interfere with the complement activation pathway. Findings in this study are discussed in the context of expanding our understanding of tick proteins that control bloodmeal feeding and hence tick-borne disease transmission by ticks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.