ArticlePloS one2013
Insulin regulates Glut4 confinement in plasma membrane clusters in adipose cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Does GLUT4 Queue? A Mechanistic Mathematical Model for Insulin Response in Adipocytes.Bulletin of mathematical biology · 2025Article
- GLUT4 dispersal at the plasma membrane of adipocytes: a super-resolved journey.Bioscience reports · 2023Review
- Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle.eLife · 2023Article
- Benchmarking Thiolate-Driven Photoswitching of Cyanine Dyes.The journal of physical chemistry. B · 2023Article
- GLUT4 translocation and dispersal operate in multiple cell types and are negatively correlated with cell size in adipocytes.Scientific reports · 2022Article
- EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes.Bioscience reports · 2022Article
- Contributive Role of Hyperglycemia and Hypoglycemia Towards the Development of Alzheimer's Disease.Molecular neurobiology · 2022Review
- Characterization of lipoprotein lipase storage vesicles in 3T3-L1 adipocytes.Journal of cell science · 2022Article
- GLUT4 On the move.The Biochemical journal · 2022Article
- Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores.International journal of molecular sciences · 2020Article
- Article
- Functional Characterization of Facilitative Glucose Transporter 4 With a Delay Responding to Plasma Glucose Level in Blunt Snout Bream (Frontiers in physiology · 2020Article
- Curculigoside and polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome improved glucose uptake via potential mTOR/AKT activated GLUT4 translocation.Journal of food and drug analysis · 2018Article
- Subcutaneous adipose tissue imaging of human obesity reveals two types of adipocyte membranes: Insulin-responsive and -nonresponsive.The Journal of biological chemistry · 2018Article
- RalA controls glucose homeostasis by regulating glucose uptake in brown fat.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A systems biology analysis connects insulin receptor signaling with glucose transporter translocation in rat adipocytes.The Journal of biological chemistry · 2017Article
- Fluorogenic probes reveal a role of GLUT4 N-glycosylation in intracellular trafficking.Nature chemical biology · 2016Article
- Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains.Scientific reports · 2016Article
- Human adipose cells in vitro are either refractory or responsive to insulin, reflecting host metabolic state.PloS one · 2015Article
- It's what's inside that matters.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Insulin-stimulated delivery of glucose transporter-4 (GLUT4) to the plasma membrane (PM) is the hallmark of glucose metabolism. In this study we examined insulin's effects on GLUT4 organization in PM of adipose cells by direct microscopic observation of single monomers tagged with photoswitchable fluorescent protein. In the basal state, after exocytotic delivery only a fraction of GLUT4 is dispersed into the PM as monomers, while most of the GLUT4 stays at the site of fusion and forms elongated clusters (60-240 nm). GLUT4 monomers outside clusters diffuse freely and do not aggregate with other monomers. In contrast, GLUT4 molecule collision with an existing cluster can lead to immediate confinement and association with that cluster. Insulin has three effects: it shifts the fraction of dispersed GLUT4 upon delivery, it augments the dissociation of GLUT4 monomers from clusters ∼3-fold and it decreases the rate of endocytic uptake. All together these three effects of insulin shift most of the PM GLUT4 from clustered to dispersed states. GLUT4 confinement in clusters represents a novel kinetic mechanism for insulin regulation of glucose homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.