ArticlePloS one2013
Nonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strain.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives.Frontiers in molecular biosciences · 2026Article
- Advancing Genetically Encoded Lysine (GEK) Chemistry: From Precision Modulation to Therapeutic Innovation.Accounts of chemical research · 2025Review
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- BeyondChemical reviews · 2025Review
- Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAs.Nucleic acids research · 2024Article
- Directed Evolution ofACS bio & med chem Au · 2024Article
- Development of orthogonal aminoacyl-tRNA synthetase mutant for incorporating a non-canonical amino acid.AMB Express · 2024Article
- Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers.Chemical reviews · 2024Review
- Site-specific dual encoding and labeling of proteins via genetic code expansion.Cell chemical biology · 2023Review
- Making Sense of "Nonsense" and More: Challenges and Opportunities in the Genetic Code Expansion, in the World of tRNA Modifications.International journal of molecular sciences · 2022Review
- Directed Evolution ofFrontiers in molecular biosciences · 2022Article
- Expanding the Genetic Code for Neuronal Studies.Chembiochem : a European journal of chemical biology · 2020Review
- Rapid Identification of Functional Pyrrolysyl-tRNA Synthetases via Fluorescence-Activated Cell Sorting.International journal of molecular sciences · 2018Article
- Therapeutic applications of genetic code expansion.Synthetic and systems biotechnology · 2018Review
- Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins.RSC advances · 2018Article
- A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins.Scientific reports · 2018Article
- Genetically Incorporating Two Distinct Post-translational Modifications into One Protein Simultaneously.ACS synthetic biology · 2018Article
- Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells.Nucleic acids research · 2018Article
- Expanding the Genetic Code ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Systematic studies of nonsense and sense suppression of the original and three derivative Methanosarcina mazei PylRS-tRNA(Pyl) pairs and cross recognition between nonsense codons and various tRNA(Pyl) anticodons in the Escherichia coli BL21(DE3) cell strain are reported. tRNA(CUA)(Pyl) is orthogonal in E. coli and able to induce strong amber suppression when it is co-expressed with pyrrolysyl-tRNA synthetase (PylRS) and charged with a PylRS substrate, N(ε)-tert-butoxycarbonyl-L-lysine (BocK). Similar to tRNA(CUA)(Pyl), tRNA(UUA)(Pyl) is also orthogonal in E. coli and can be coupled with PylRS to genetically incorporate BocK at an ochre mutation site. Although tRNA(UUA)(Pyl) is expected to recognize a UAG codon based on the wobble hypothesis, the PylRS-tRNA(UUA)(Pyl) pair does not give rise to amber suppression that surpasses the basal amber suppression level in E. coli. E. coli itself displays a relatively high opal suppression level and tryptophan (Trp) is incorporated at an opal mutation site. Although the PylRS-tRNA(UCA)(Pyl) pair can be used to encode BocK at an opal codon, the pair fails to suppress the incorporation of Trp at the same site. tRNA(CCU)(Pyl) fails to deliver BocK at an AGG codon when co-expressed with PylRS in E. coli.
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