ArticleAdvanced functional materials2011
Polymeric Aqueous Biphasic System Rehydration Facilitates High Throughput Cell Exclusion Patterning For Cell Migration Studies.
Article in Advanced functional materials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Automated Analysis of Extracellular Matrix Invasion of Cancer Cells from Tumor Spheroids.ACS measurement science au · 2024Article
- Thymoquinone Alterations of the Apoptotic Gene Expressions and Cell Cycle Arrest in Genetically Distinct Triple-Negative Breast Cancer Cells.Nutrients · 2022Article
- Protocell arrays for simultaneous detection of diverse analytes.Nature communications · 2021Article
- Phytochemicals inhibit migration of triple negative breast cancer cells by targeting kinase signaling.BMC cancer · 2020Article
- Confinement of Suspension-Cultured Cells in Polyethylene Glycol/Polyethylene Oxide-Albumin Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Stigmatic Microscopy Enables Low-Cost, 3D, Microscale Particle Imaging Velocimetry in Rehydrating Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Biomaterials-Based Approaches to Tumor Spheroid and Organoid Modeling.Advanced healthcare materials · 2018Review
- Liquid-based three-dimensional tumor models for cancer research and drug discovery.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Evaporative edge lithography of a liposomal drug microarray for cell migration assays.Nanofabrication · 2015Article
- Phytochemicals potently inhibit migration of metastatic breast cancer cells.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Droplet formation and shrinking in aqueous two-phase systems using a membrane emulsification method.Biomicrofluidics · 2015Article
- Robotic production of cancer cell spheroids with an aqueous two-phase system for drug testing.Journal of visualized experiments : JoVE · 2015Article
- High Throughput, Polymeric Aqueous Two-Phase Printing of Tumor Spheroids.Advanced functional materials · 2014Article
- Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-Cancer Drug Testing in 3D Culture.Cellular and molecular bioengineering · 2014Article
- Cell co-culture patterning using aqueous two-phase systems.Journal of visualized experiments : JoVE · 2013Article
- Rapid generation of multiplexed cell cocultures using acoustic droplet ejection followed by aqueous two-phase exclusion patterning.Tissue engineering. Part C, Methods · 2012Article
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6 authors.
Funding
Abstract
This paper describes a cell-exclusion patterning method facilitated by a polymeric aqueous two-phase system. The immersion aqueous phase (polyethylene glycol) containing cells rehydrates a dried disk of the denser phase (dextran) on the substrate to form a dextran droplet. With the right properties of the phase-forming polymers, the rehydrating droplet remains immiscible with the immersion phase. Proper formulation of the two-phase system ensures that the interfacial tension between the rehydrating droplet and the surrounding aqueous phase prevents cells from crossing the interface so that cells only adhere to the regions of the substrate around the dextran phase droplet. Washing out the patterning two-phase reagents reveals a cell monolayer containing a well-defined circular gap that serves as the migration niche for cells of the monolayer. Migration of cells into the cell-excluded area is readily visualized and quantified over time. A 96-well plate format of this "gap healing" migration assay demonstrates the ability to detect inhibition of cell migration by known cytoskeleton targeting agents. This straightforward method, which only requires a conventional liquid handler and readily prepared polymer solutions, opens new opportunities for high throughput cell migration assays.
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