ArticleThe Journal of membrane biology2013
3D spheroids' sensitivity to electric field pulses depends on their size.
Article in The Journal of membrane biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a.Translational oncology · 2026Article
- Characterization of glioma spheroid viability and metastatic potential following monophasic and biphasic pulsed electric fields.Bioelectrochemistry (Amsterdam, Netherlands) · 2025Article
- The Efficacy of Electrochemotherapy with Dacarbazine on Melanoma Cells.Bioelectricity · 2024Article
- Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages.Cancers · 2020Article
- Evaluations of Acute and Sub-Acute Biological Effects of Narrowband and Moderate-Band High Power Electromagnetic Waves on Cellular Spheroids.Scientific reports · 2019Article
- 3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.Lab on a chip · 2019Article
- Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium.Scientific reports · 2019Article
- Upregulation of DNA Sensors in B16.F10 Melanoma Spheroid Cells After Electrotransfer of pDNA.Technology in cancer research & treatment · 2018Article
- Recellularized human dermis for testing gene electrotransfer ex vivo.Biomedical materials (Bristol, England) · 2016Article
- Calcium Electroporation: Evidence for Differential Effects in Normal and Malignant Cell Lines, Evaluated in a 3D Spheroid Model.PloS one · 2015Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dramatic differences of cells behavior exist between cells cultured under classical 2D monolayers and 3D models, the latter being closer to in vivo responses. Thus, many 3D cell culture models have been developed. Among them, multicellular tumor spheroid appears as a nice and easy-to-handle 3D model based on cell adhesion properties. It is composed of one or several cell types and is widely used to address carcinogenesis, or drugs screening. A few and recent publications report the use of spheroids to investigate electropermeabilization process. We studied the response of spheroids to electrical field pulses (EP) in terms of their age, diameter or formation technique. We found that small human HCT-116 colorectal spheroids are more sensitive to electric field pulses than larger ones. Indeed, the growth of spheroids with a diameter of 300 μm decreased by a factor 2 over 4 days when submitted to EP (8 pulses, lasting 100 μs at a 1,300 V/cm field intensity). Under those electrical conditions, 650 μm spheroids were not affected. These data were the same whatever the formation method (i.e. hanging drop and nonadherent techniques). These observations point out the fact that characteristics of 3D cell models have to be taken into account to avoid biased conclusions of experimental data.
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