ArticleBiology open2013
Effective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis.
Article in Biology open, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Review
- A Glimpse of the Peptide Profile Presentation byJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Impacts of the MHC class I-like XNC10 and innate-like T cells on tumor tolerance and rejection in the amphibian Xenopus.Carcinogenesis · 2019Article
- Nucleated red blood cells: Immune cell mediators of the antiviral response.PLoS pathogens · 2018Article
- Frog's DCs have it all in one.European journal of immunology · 2018Article
- Frog Skin Innate Immune Defences: Sensing and Surviving Pathogens.Frontiers in immunology · 2018Review
- Tumor immunology viewed from alternative animal models-theCurrent pathobiology reports · 2017Article
- Nonclassical MHC-Restricted Invariant Vα6 T Cells Are Critical for Efficient Early Innate Antiviral Immunity in the Amphibian Xenopus laevis.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Nonclassical MHC class I-dependent invariant T cells are evolutionarily conserved and prominent from early development in amphibians.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology. We generated transgenic outbred X. laevis and isogenetic laevis/gilli cloned lines with stably silenced expression of β2-microglobulin (b2m) critical for class I function. Transgenic F1 frogs exhibited decreased surface class I expression on erythrocytes and lymphocytes, decreased frequency of peripheral CD8 T cells and impaired CD8 T cell-mediated skin allograft rejection. Additionally, b2m knockdown increased susceptibility to viral infection of F0 transgenic larvae. This loss of function strategy offers new avenues for studying ontogeny of immunity and other developmental processes in Xenopus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.