ArticleBiology open2013
Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant.
Article in Biology open, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix.The Journal of cell biology · 2020Article
- Review
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Mitotic spindle assembly in animal cells: a fine balancing act.Nature reviews. Molecular cell biology · 2017Review
- The Seckel syndrome and centrosomal protein Ninein localizes asymmetrically to stem cell centrosomes but is not required for normal development, behavior, or DNA damage response in Drosophila.Molecular biology of the cell · 2016Article
- Centrosomes are multifunctional regulators of genome stability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells.The Journal of cell biology · 2015Article
- p53 protects against genome instability following centriole duplication failure.The Journal of cell biology · 2015Article
- Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo.PLoS genetics · 2015Article
- Acentrosomal Drosophila epithelial cells exhibit abnormal cell division, leading to cell death and compensatory proliferation.Developmental cell · 2014Article
- Cell lines.Methods (San Diego, Calif.) · 2014Review
- Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Loss of centrioles causes chromosomal instability in vertebrate somatic cells.The Journal of cell biology · 2013Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In animal cells the centrosome is commonly viewed as the main cellular structure driving microtubule (MT) assembly into the mitotic spindle apparatus. However, additional pathways, such as those mediated by chromatin and augmin, are involved in the establishment of functional spindles. The molecular mechanisms involved in these pathways remain poorly understood, mostly due to limitations inherent to current experimental systems available. To overcome these limitations we have developed six new Drosophila cell lines derived from Drosophila homozygous mutants for DSas-4, a protein essential for centriole biogenesis. These cells lack detectable centrosomal structures, astral MT, with dispersed pericentriolar proteins D-PLP, Centrosomin and γ-tubulin. They show poorly focused spindle poles that reach the plasma membrane. Despite being compromised for functional centrosome, these cells could successfully undergo mitosis. Live-cell imaging analysis of acentriolar spindle assembly revealed that nascent MTs are nucleated from multiple points in the vicinity of chromosomes. These nascent MTs then grow away from kinetochores allowing the expansion of fibers that will be part of the future acentriolar spindle. MT repolymerization assays illustrate that acentriolar spindle assembly occurs "inside-out" from the chromosomes. Colchicine-mediated depolymerization of MTs further revealed the presence of a functional Spindle Assembly Checkpoint (SAC) in the acentriolar cells. Finally, pilot RNAi experiments open the potential use of these cell lines for the molecular dissection of anastral pathways in spindle and centrosome assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.