ArticleSpringerPlus2012
Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn's disease-type necrotizing colitis in Mice.
Article in SpringerPlus, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Development of visual dye-based loop-mediated isothermal amplification assays forJournal of veterinary science · 2025Article
- Mycobacterium avium subsp. paratuberculosis and Crohn's disease: characterization of the interaction with different aspects of the disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Development and evaluation of LAMP-coupled lateral flow device for the detection of MAP in livestock at point of care resource-limited areas.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Exploring the potential hazard ofVeterinary world · 2017Article
- Article
- Detection of Mycobacteria by Culture and DNA-Based Methods in Animal-Derived Food Products Purchased at Spanish Supermarkets.Frontiers in microbiology · 2017Article
- Detection of Mycobacterium avium subspecies paratuberculosis in tie-stall dairy herds using a standardized environmental sampling technique and targeted pooled samples.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- Influence of environmental factors in the development of inflammatory bowel diseases.World journal of gastrointestinal pharmacology and therapeutics · 2016Review
- Role of the gut microbiota in inflammatory bowel disease pathogenesis: what have we learnt in the past 10 years?World journal of gastroenterology · 2014Review
- Multi-scale optical imaging of the delayed type hypersensitivity reaction attenuated by rapamycin.Theranostics · 2014Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced murine colitis model was developed to investigate the pathogenesis and to evaluate a method of treating human Crohn's disease. This experimental model rapidly induces colitis similar to human Crohn's disease lesion in a reproducible manner. However, natural exposure of the human digestive tract to TNBS is unrealistic. A novel animal model based on realistic data is eagerly anticipated in future research on pathogenesis of CD.
methodWe evaluated the potency of Map antigen molecules in an effort to develop a novel colitis model using a more realistic source than TNBS. We prepared the Map antigen by ethanol extraction and developed a mouse model in a manner similar to that of the well-known TNBS-induced colitis in mice. In the experiment, seven days after subcutaneous (SC) injection of the antigen into normal C57BL/6 mice, the same antigen in 50% ethanol was injected into the colon by the transanal route with a fine cannula.
resultsOn the fifth day after the transanal injection, histopathological examination revealed full-thickness necrotizing colitis with erosion and ulcers; severe infiltration with neutrophils, lymphocytes, macrophages, and perforation. However, no change was detected with each single Map-antigen injection.
conclusionThe present results provide a novel animal model for research on CD and may be the key to clarifying the relationship between CD and Map. This is the first evidence that mycobacterium antigen induces necrotizing colitis.
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