ArticleInvestigative ophthalmology & visual science2013
Effect of VIP on intracellular [Ca2+], extracellular regulated kinase 1/2, and secretion in cultured rat conjunctival goblet cells.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Role of tear vasoactive intestinal peptide on dry eyes after laser keratorefractive surgery.BMC ophthalmology · 2023Trial
- Purinergic agonists increase [CaAmerican journal of physiology. Cell physiology · 2024Article
- Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators.Scientific reports · 2022Article
- Signaling Pathways Used by the Specialized Pro-Resolving Mediator Maresin 2 Regulate Goblet Cell Function: Comparison with Maresin 1.International journal of molecular sciences · 2022Article
- Immunity and pain in the eye: focus on the ocular surface.Clinical and experimental immunology · 2022Review
- The G Protein-Coupled Receptor, VPAC1, Mediates Vasoactive Intestinal Peptide-Dependent Functional Homeostasis of the Gut Microbiota.Gastro hep advances · 2022Article
- H1-Receptor Antagonist Olopatadine Inhibits MUC5AC Secretion by Conjunctival Goblet Cells.Bulletin of experimental biology and medicine · 2021Article
- Impact of P2XInternational journal of molecular sciences · 2021Article
- Maresin 1, a specialized proresolving mediator, stimulates intracellular [CaJournal of cellular physiology · 2021Article
- Pro-Resolving Mediator Annexin A1 Regulates Intracellular CaFrontiers in immunology · 2021Article
- Bioelectric Responses of Conjunctival Goblet Cells to Dry Eye: Impact of Ion Channels on Exocytotic Function and Viability.International journal of molecular sciences · 2020Article
- VIP and muscarinic synergistic mucin secretion by salivary mucous cells is mediated by enhanced PKC activity via VIP-induced release of an intracellular CaPflugers Archiv : European journal of physiology · 2020Article
- Resolvin D2 elevates cAMP to increase intracellular [CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- VPAC1 couples with TRPV4 channel to promote calcium-dependent gastric cancer progression via a novel autocrine mechanism.Oncogene · 2019Article
- Immunoresolvent Resolvin D1 Maintains the Health of the Ocular Surface.Advances in experimental medicine and biology · 2019Review
- Role of ion channels in the functional response of conjunctival goblet cells to dry eye.American journal of physiology. Cell physiology · 2018Article
- TFOS DEWS II pain and sensation report.The ocular surface · 2017Review
- VPAC Receptor Subtypes Tune Purinergic Neuron-to-Glia Communication in the Murine Submucosal Plexus.Frontiers in cellular neuroscience · 2017Article
- Neither non-toxigenicBMJ open ophthalmology · 2017Article
- Lipoxin AMucosal immunology · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo determine the intracellular signaling pathways that vasoactive intestinal peptide (VIP) uses to stimulate high molecular weight glycoconjugate secretion from cultured rat conjunctival goblet cells.
methodsGoblet cells from rat bulbar and forniceal conjunctiva were grown in organ culture. Presence and localization of VIP receptors (VPAC1 and 2) were determined by RT-PCR, immunofluorescence microscopy and Western blot analysis. Intracellular [Ca(2+)] ([Ca(2+)]i) was measured using fura-2. Extracellular signal-regulated kinase (ERK)-1/2 activity was determined by Western blot analysis. High molecular weight glycoconjugate secretion was measured with an enzyme-linked lectin assay on cultured goblet cells that were serum-starved for 2 hours before stimulation with VIP, VPAC1-, or VPAC2-specific agonists. Inhibitors were added 30 minutes prior to VIP. Activation of epidermal growth factor receptor (EGFR) was measured by immunoprecipitation using an antibody against pTyr followed by Western blot analysis with an antibody against EGFR.
resultsBoth VIP receptors were present in rat conjunctiva and cultured goblet cells. VIP- and VPAC-specific agonists increased [Ca(2+)]i and secretion in a concentration-dependent manner. VIP also increased ERK1/2 activity, VIP-stimulated increase in [Ca(2+)]i. Secretion, but not ERK1/2 activity, was inhibited by the protein kinase A inhibitor, H89. VIP-stimulated secretion was inhibited by siRNA for ERK2 but not by siRNA for EGFR. VIP did not increase the phosphorylation of the EGFR.
conclusionsIn conclusion, in cultured rat conjunctival goblet cells, VPAC1 and 2 receptors are functional. VIP stimulates a cAMP-dependent increase in [Ca(2+)]i and glycoconjugate secretion, but not ERK1/2 activation. VIP does not activate with EGFR.
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