ArticleNature protocols2013
DNA nanotubes for NMR structure determination of membrane proteins.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- DNA Helix Bundle-Encoded Multi-Bit Information Readout by Sapphire-Supported Nanopores.Advanced functional materials · 2026Article
- Recycling Materials for Sustainable DNA Origami Manufacturing.Nano letters · 2024Article
- Visualization of the hepatic and renal cell uptake and trafficking of tetrahedral DNA origami in tumour.Cell proliferation · 2024Article
- DNA Origami: Recent Progress and Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Recent Progress of Magnetically Actuated DNA Micro/Nanorobots.Cyborg and bionic systems (Washington, D.C.) · 2022Review
- DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.Nano letters · 2020Article
- Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation.ACS applied materials & interfaces · 2019Article
- Article
- Autonomously designed free-form 2D DNA origami.Science advances · 2019Article
- Opportunities and Challenges of Backbone, Sidechain, and RDC Experiments to Study Membrane Protein Dynamics in a Detergent-Free Lipid Environment Using Solution State NMR.Frontiers in molecular biosciences · 2019Review
- Design and operation of reconfigurable two-dimensional DNA molecular arrays.Nature protocols · 2018Article
- Article
- Article
- Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs.Journal of biomolecular NMR · 2015Article
- Assembly of RNA nanostructures on supported lipid bilayers.Nanoscale · 2015Article
- Facile and scalable preparation of pure and dense DNA origami solutions.Angewandte Chemie (International ed. in English) · 2014Article
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins.Nucleic acids research · 2014Article
- DNA origami-based standards for quantitative fluorescence microscopy.Nature protocols · 2014Article
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Authors and funding
4 authors.
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Abstract
Finding a way to determine the structures of integral membrane proteins using solution nuclear magnetic resonance (NMR) spectroscopy has proved to be challenging. A residual-dipolar-coupling-based refinement approach can be used to resolve the structure of membrane proteins up to 40 kDa in size, but to do this you need a weak-alignment medium that is detergent-resistant and it has thus far been difficult to obtain such a medium suitable for weak alignment of membrane proteins. We describe here a protocol for robust, large-scale synthesis of detergent-resistant DNA nanotubes that can be assembled into dilute liquid crystals for application as weak-alignment media in solution NMR structure determination of membrane proteins in detergent micelles. The DNA nanotubes are heterodimers of 400-nm-long six-helix bundles, each self-assembled from a M13-based p7308 scaffold strand and >170 short oligonucleotide staple strands. Compatibility with proteins bearing considerable positive charge as well as modulation of molecular alignment, toward collection of linearly independent restraints, can be introduced by reducing the negative charge of DNA nanotubes using counter ions and small DNA-binding molecules. This detergent-resistant liquid-crystal medium offers a number of properties conducive for membrane protein alignment, including high-yield production, thermal stability, buffer compatibility and structural programmability. Production of sufficient nanotubes for four or five NMR experiments can be completed in 1 week by a single individual.
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