ArticleProteome science2013
Pre-analytical stability of the plasma proteomes based on the storage temperature.
Article in Proteome science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The effect of preanalytical factors on cerebrospinal fluid and plasma proteomics: a systematic experimental study.Clinical proteomics · 2026Article
- Sex-specific association between low oral doses of cannabidiol (CBD) and plasma concentration of anandamide (AEA), N-palmitoylethanolamine (PEA) and N-oleoylethanolamine (OEA) in healthy occasional cannabis users.Journal of cannabis research · 2026Article
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- Assessing the causal relationship between plasma proteins and osteoporosis: novel insights into pathological mechanisms and therapeutic implications.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2024Article
- The Impact of Acute Nutritional Interventions on the Plasma Proteome.The Journal of clinical endocrinology and metabolism · 2023Article
- Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood.Biomedicines · 2022Article
- Effect of serum sample storage temperature on metabolomic and proteomic biomarkers.Scientific reports · 2022Article
- Serum transthyretin and risk of cognitive decline and dementia: 22-year longitudinal study.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2021Article
- An Exploratory Pilot Study with Plasma Protein Signatures Associated with Response of Patients with Depression to Antidepressant Treatment for 10 Weeks.Biomedicines · 2020Article
- Stability of the Human Plasma Proteome to Pre-analytical Variability as Assessed by an Aptamer-Based Approach.Journal of proteome research · 2019Article
- Effect of Repeated Freeze-Thaw on Serum Biomarkers Associated with Eye Disease.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer's disease diagnostics.Scientific reports · 2017Article
- Ex vivo instability of glycated albumin: A role for autoxidative glycation.Archives of biochemistry and biophysics · 2017Article
- The Utility of Acute-Phase Proteins in the Assessment of Treatment Response in Dogs With Bacterial Pneumonia.Journal of veterinary internal medicine · 2017Observational
- Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds.Biotechnology progress · 2016Article
- Phenotype-Driven Plasma Biobanking Strategies and Methods.Journal of personalized medicine · 2015Article
- Exposures of Sus scrofa to a TASER(®) conducted electrical weapon: no effects on 2-dimensional gel electrophoresis patterns of plasma proteins.Forensic science, medicine, and pathology · 2014Article
- Experimental study on the viscosity and adhesive performance of exogenous liquid fibrin glue.Neurologia medico-chirurgica · 2014Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study examined the effect of storage temperature on the protein profile of human plasma. Plasma samples were stored for 13 days at -80°C, -20°C, +4°C and room temperature (20-25°C) prior to proteomic analysis. The proteomic comparisons were based on the differences of mean intensity values of protein spots between fresh plasma samples (named "time zero") and plasma samples stored at different temperatures. To better understand the thermally induced biochemical changes that may affect plasma proteins during storage we identified proteins with different expressions with respect to the time zero sample.
resultsUsing two-dimensional electrophoresis followed by MALDI-TOF MS and /or LC-MS/MS 20 protein spots representing 10 proteins were identified with significant differences in abundance when stored at different temperatures. Our results, in agreement with various authors, indicate that during storage for a short period (13 days) at four different temperatures plasma proteins were more affected by degradation processes at +4°C compared to the other temperatures analysed. However, we founded that numerous protein spots (vitamin D binding protein, alpha-1-antitrypsin, serotransferrin, apoplipoprotein A-I, apolipoprotein E, haptoglobin and complement factor B) decrease in abundance with increasing temperature up to 4°C, but at room temperature their intensity mean values are similar to those of time zero and -80°C. We hypothesize that these proteins are labile at 4°C, but at the same time they are stable at room temperature (20-25°C). Furthermore we have grouped the proteins based on their different sensitivity to the storage temperature. Spots of serum albumin, fibrinogen gamma chain and haptoglobin are more resistant to the higher temperatures tested, as they have undergone changes in abundance only at room temperature; conversely, other spots of serum albumin, fibrinogen beta chain and serotransferrin are more labile as they have undergone changes in abundance at all temperatures except at -80°C.
conclusionsAlthough there are many studies concerning protein stability of clinical samples during storage these findings may help to provide a better understanding of the changes of proteins induced by storage temperature.
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Registered trials
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