ArticleToxicology in vitro : an international journal published in association with BIBRA2013
Nitroxide TEMPO: a genotoxic and oxidative stress inducer in cultured cells.
Article in Toxicology in vitro : an international journal published in association with BIBRA, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Nitroxides: Chemistry, Antioxidant Properties, and Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Effect of Low Concentration of Nitroxides on SH-SY5Y Cells Transfected with the Tau Protein.International journal of molecular sciences · 2023Article
- Spatial-Selective Volumetric 4D Printing and Single-Photon Grafting of Biomolecules within Centimeter-Scale Hydrogels via Tomographic Manufacturing.Advanced materials technologies · 2023Article
- The role of trace N-Oxyl compounds as redox mediator in enhancing antiviral ribavirin elimination in UV/Chlorine process.Applied catalysis. B, Environmental · 2022Article
- Optimized Photoclick (Bio)Resins for Fast Volumetric Bioprinting.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y TkMethods in molecular biology (Clifton, N.J.) · 2020Article
- Benchmark Dose Modeling ofToxicological research · 2018Review
- Comparative Genotoxicity of TEMPO and 3 of Its Derivatives in Mouse Lymphoma Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- ROS generation and JNK activation contribute to 4-methoxy-TEMPO-induced cytotoxicity, autophagy, and DNA damage in HepG2 cells.Archives of toxicology · 2018Article
- Nitroxides as Antioxidants and Anticancer Drugs.International journal of molecular sciences · 2017Review
- Reactive oxygen species and c-Jun N-terminal kinases contribute to TEMPO-induced apoptosis in L5178Y cells.Chemico-biological interactions · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
2,2,6,6-Tetramethylpiperidine-1-oxyl (TEMPO) is a low molecular weight nitroxide and stable free radical. In this study, we investigated the cytotoxicity and genotoxicity of TEMPO in mammalian cells using the mouse lymphoma assay (MLA) and in vitro micronucleus assay. In the absence of metabolic activation (S9), 3mM TEMPO produced significant cytotoxicity and marginal mutagenicity in the MLA; in the presence of S9, treatment of mouse lymphoma cells with 1-2mM TEMPO resulted in dose-dependent decreases of the relative total growth and increases in mutant frequency. Treatment of TK6 human lymphoblastoid cells with 0.9-2.3mM TEMPO increased the frequency of both micronuclei (a marker for clastogenicity) and hypodiploid nuclei (a marker of aneugenicity) in a dose-dependent manner; greater responses were produced in the presence of S9. Within the dose range tested, TEMPO induced reactive oxygen species and decreased glutathione levels in mouse lymphoma cells. In addition, the majority of TEMPO-induced mutants had loss of heterozygosity at the Tk locus, with allele loss of ⩽34Mbp. These results indicate that TEMPO is mutagenic in the MLA and induces micronuclei and hypodiploid nuclei in TK6 cells. Oxidative stress may account for part of the genotoxicity induced by TEMPO in both cell lines.
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Registered trials
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