ArticleMolecular microbiology2013
The adhesive and cohesive properties of a bacterial polysaccharide adhesin are modulated by a deacetylase.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Analysis of biofilm and bacterial communities in the towel environment with daily use.Scientific reports · 2023Article
- HfaE Is a Component of the Holdfast Anchor Complex That Tethers the Holdfast Adhesin to the Cell Envelope.Journal of bacteriology · 2022Article
- Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens.Molecular microbiology · 2022Article
- A polysaccharide deacetylase enhances bacterial adhesion in high-ionic-strength environments.iScience · 2021Article
- Comparative Analysis of Ionic Strength Tolerance between Freshwater and MarineJournal of bacteriology · 2019Article
- Composition of the Holdfast Polysaccharide fromJournal of bacteriology · 2019Article
- A Genome-Wide Analysis of Adhesion inmBio · 2019Article
- Feedback regulation of Caulobacter crescentus holdfast synthesis by flagellum assembly via the holdfast inhibitor HfiA.Molecular microbiology · 2018Article
- Microbial Extracellular Polymeric Substances: Ecological Function and Impact on Soil Aggregation.Frontiers in microbiology · 2018Review
- Role of de-N-acetylase PgaB from Aggregatibacter actinomycetemcomitans in exopolysaccharide export in biofilm mode of growth.Molecular oral microbiology · 2017Article
- Cohesive Properties of themBio · 2017Article
- A Rhizobiales-Specific Unipolar Polysaccharide Adhesin Contributes to Rhodopseudomonas palustris Biofilm Formation across Diverse Photoheterotrophic Conditions.Applied and environmental microbiology · 2017Article
- Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane.The Journal of biological chemistry · 2016Article
- Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria.Microbiology spectrum · 2015Review
- P. aeruginosa SGNH hydrolase-like proteins AlgJ and AlgX have similar topology but separate and distinct roles in alginate acetylation.PLoS pathogens · 2014Article
- Sequential evolution of bacterial morphology by co-option of a developmental regulator.Nature · 2014Article
- Draft Genome Sequence of the Dimorphic Prosthecate Bacterium Brevundimonas abyssalis TAR-001T.Genome announcements · 2013Article
- Physiochemical properties of Caulobacter crescentus holdfast: a localized bacterial adhesive.The journal of physical chemistry. B · 2013Article
- Function and Regulation of Agrobacterium tumefaciens Cell Surface Structures that Promote Attachment.Current topics in microbiology and immunologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial exopolysaccharide synthesis is a prevalent and indispensible activity in many biological processes, including surface adhesion and biofilm formation. In Caulobacter crescentus, surface attachment and subsequent biofilm growth depend on the ability to synthesize an adhesive polar polysaccharide known as the holdfast. In this work, we show that polar polysaccharide synthesis is a conserved phenomenon among Alphaproteobacterial species closely related to C. crescentus. Among them, mutagenesis of Asticcacaulis biprosthecum showed that disruption of the hfsH gene, which encodes a putative polysaccharide deacetylase, leads to accumulation of holdfast in the culture supernatant. Examination of the hfsH deletion mutant in C. crescentus revealed that this strain synthesizes holdfast; however, like the A. biprosthecum hfsH mutant, the holdfasts are shed into the medium and have decreased adhesiveness and cohesiveness. Site-directed mutagenesis at the predicted catalytic site of C. crescentus HfsH phenocopied the ΔhfsH mutant and abolished the esterase activity of HfsH. In contrast, overexpression of HfsH increased cell adherence without increasing holdfast synthesis. We conclude that the polysaccharide deacetylase activity of HfsH is required for the adhesive and cohesive properties of the holdfast, as well as for the anchoring of the holdfast to the cell envelope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.