ArticleMolecular microbiology2013
Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Selective sugar transport supports Proteus mirabilis fitness in the urinary tract.PLoS pathogens · 2026Article
- An Escherichia coli Phosphotransferase System Modulates Methylglyoxal Resistance by Regulating Intracellular Potassium.Molecular microbiology · 2026Article
- Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.Journal of bacteriology · 2026Review
- Article
- Fructose-responsive regulation by FruR in Faecalibacterium prausnitzii for its intestinal colonization.Communications biology · 2025Article
- PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes.Journal of bacteriology · 2023Article
- Regulation of phosphate starvation-specific responses inMicrobiology (Reading, England) · 2023Review
- GTPase Era at the heart of ribosome assembly.Frontiers in molecular biosciences · 2023Review
- The role of nitrogen-responsive regulators in controlling inorganic polyphosphate synthesis inMicrobiology (Reading, England) · 2022Article
- Fosfomycin Resistance Evolutionary Pathways ofInternational journal of molecular sciences · 2022Article
- Article
- Cell cycle control and environmental response by second messengers in Caulobacter crescentus.BMC bioinformatics · 2020Article
- Global control of bacterial nitrogen and carbon metabolism by a PTSProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Modification of Regulatory Circuits Involved in the Control of Polyhydroxyalkanoates Metabolism to Improve Their Production.Frontiers in bioengineering and biotechnology · 2020Review
- Model of a Kinetically Driven Crosstalk between Paralogous Protein Encounter Complexes.Biophysical journal · 2019Article
- Regulation of Iron Uptake by Fine-Tuning the Iron Responsiveness of the Iron Sensor Fur.Applied and environmental microbiology · 2019Article
- Article
- Determination of protein phosphorylation by polyacrylamide gel electrophoresis.Journal of microbiology (Seoul, Korea) · 2019Article
- Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain.Nucleic acids research · 2019Article
- Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
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Abstract
In addition to the phosphoenolpyruvate:sugar phosphotransferase system (sugar PTS), most proteobacteria possess a paralogous system (nitrogen phosphotransferase system, PTS(Ntr)). The first proteins in both pathways are enzymes (enzyme I(sugar) and enzyme I(Ntr)) that can be autophosphorylated by phosphoenolpyruvate. The most striking difference between enzyme I(sugar) and enzyme I(Ntr) is the presence of a GAF domain at the N-terminus of enzyme I(Ntr). Since the PTS(Ntr) was identified in 1995, it has been implicated in a variety of cellular processes in many proteobacteria and many of these regulations have been shown to be dependent on the phosphorylation state of PTS(Ntr) components. However, there has been little evidence that any component of this so-called PTS(Ntr) is directly involved in nitrogen metabolism. Moreover, a signal regulating the phosphorylation state of the PTS(Ntr) had not been uncovered. Here, we demonstrate that glutamine and α-ketoglutarate, the canonical signals of nitrogen availability, reciprocally regulate the phosphorylation state of the PTS(Ntr) by direct effects on enzyme I(Ntr) autophosphorylation and the GAF signal transduction domain is necessary for the regulation of enzyme I(Ntr) activity by the two signal molecules. Taken together, our results suggest that the PTS(Ntr) senses nitrogen availability.
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