ArticleBMC microbiology2013
German Francisella tularensis isolates from European brown hares (Lepus europaeus) reveal genetic and phenotypic diversity.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 54 citations in OpenAlex.
- [Zoonoses in endemic, free-ranging mammals].Pathologie (Heidelberg, Germany) · 2023Article
- Case report: tularaemia in a white-handed gibbon (International journal of veterinary science and medicine · 2023Article
- Phylogeography ofFrontiers in microbiology · 2023Article
- Ulceroglandular form of tularemia after squirrel bite: a case report.Journal of medical case reports · 2022Article
- Tularemia: a re-emerging tick-borne infectious disease.Folia microbiologica · 2021Review
- Genotyping ofMicroorganisms · 2020Article
- Virulence ofMicroorganisms · 2020Article
- Outbreak of Tularemia in a Group of Hunters in Germany in 2018-Kinetics of Antibody and Cytokine Responses.Microorganisms · 2020Article
- Review
- Using affinity propagation clustering for identifying bacterial clades and subclades with whole-genome sequences of Francisella tularensis.PLoS neglected tropical diseases · 2020Article
- Phylogeography and Genetic Diversity ofFrontiers in microbiology · 2020Article
- Evolution of Antibiotic Resistance in Surrogates ofFrontiers in microbiology · 2020Article
- Molecular identification of the source of an uncommon tularaemia outbreak, Germany, autumn 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Genetic Diversity and Spatial Segregation ofFrontiers in cellular and infection microbiology · 2019Article
- Phylogeographic Distribution of Human and HareFrontiers in cellular and infection microbiology · 2019Article
- RevisitingFrontiers in microbiology · 2018Article
- Population Genomics ofFrontiers in cellular and infection microbiology · 2018Article
- Phylogenetic Lineages ofFrontiers in cellular and infection microbiology · 2018Review
- Tularemia in Germany-A Re-emerging Zoonosis.Frontiers in cellular and infection microbiology · 2018Review
- High-Quality Draft Genome Sequence ofGenome announcements · 2017Article
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Authors and funding
20 authors at 9 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTularemia is a zoonotic disease caused by Francisella tularensis that has been found in many different vertebrates. In Germany most human infections are caused by contact with infected European brown hares (Lepus europaeus). The aim of this study was to elucidate the epidemiology of tularemia in hares using phenotypic and genotypic characteristics of F. tularensis.
resultsCultivation of F. tularensis subsp. holarctica bacteria from organ material was successful in 31 of 52 hares that had a positive PCR result targeting the Ft-M19 locus. 17 isolates were sensitive to erythromycin and 14 were resistant. Analysis of VNTR loci (Ft-M3, Ft-M6 and Ft-M24), INDELs (Ftind33, Ftind38, Ftind49, RD23) and SNPs (B.17, B.18, B.19, and B.20) was shown to be useful to investigate the genetic relatedness of Francisella strains in this set of strains. The 14 erythromycin resistant isolates were assigned to clade B.I, and 16 erythromycin sensitive isolates to clade B.IV and one isolate was found to belong to clade B.II. MALDI-TOF mass spectrometry (MS) was useful to discriminate strains to the subspecies level.
conclusionsF. tularensis seems to be a re-emerging pathogen in Germany. The pathogen can easily be identified using PCR assays. Isolates can also be identified within one hour using MALDI-TOF MS in laboratories where specific PCR assays are not established. Further analysis of strains requires genotyping tools. The results from this study indicate a geographical segregation of the phylogenetic clade B.I and B.IV, where B.I strains localize primarily within eastern Germany and B.IV strains within western Germany. This phylogeographical pattern coincides with the distribution of biovar I (erythromycin sensitive) and biovar II (erythromycin resistance) strains. When time and costs are limiting parameters small numbers of isolates can be analysed using PCR assays combined with DNA sequencing with a focus on genetic loci that are most likely discriminatory among strains found in a specific area. In perspective, whole genome data will have to be investigated especially when terrorist attack strains need to be tracked to their genetic and geographical sources.
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