ArticleBMC cancer2013
Targeting Id1 reduces proliferation and invasion in aggressive human salivary gland cancer cells.
Article in BMC cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- MYB fusions and CD markers as tools for authentication and purification of cancer stem cells from salivary adenoid cystic carcinoma.Stem cell research · 2017Article
- Suppression of invasion and metastasis in aggressive salivary cancer cells through targeted inhibition of ID1 gene expression.Cancer letters · 2016Article
- Id Proteins Contribute to Tumor Development and Metastatic Colonization in a Model of Bladder Carcinogenesis.Bladder cancer (Amsterdam, Netherlands) · 2015Article
- Insulin-like growth factor--phosphatidylinositol 3 kinase signaling in canine cortisol-secreting adrenocortical tumors.Journal of veterinary internal medicine · 2015Article
- TGF-β1-induced expression of Id-1 is associated with tumor progression in gastric cancer.Medical oncology (Northwood, London, England) · 2014Article
- Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis.PloS one · 2014Article
- Serum regulation of Id1 expression by a BMP pathway and BMP responsive element.Biochimica et biophysica acta · 2013Article
- A review: Immunological markers for malignant salivary gland tumors.Journal of oral biology and craniofacial researchReview
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundSalivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-helix transcription factors that control malignant cell behavior and tumor aggressiveness in many tissues. In this study, our goal was to determine the potential role of Id proteins, particularly Id1, during human SGC cell progression.
methodsWe first determined the expression levels of Id1 and Id2 in four SGC cell lines: two adenocarcinoma of the salivary gland (HSG and HSY) and two adenoid cystic carcinoma (ACC2 and ACCM) cell lines. We then used constructs that expressed antisense cDNAs to Id1 or Id2 to knockdown the expression of these proteins in cell lines where they were highly expressed, and determined the effects of the knockdown on cell proliferation, migration and invasion.
resultsId1 mRNA and protein were detectable in all cell lines, and expression of Id2 was variable, from absent to high. The ACC2 and ACCM cell lines expressed both Id1 and Id2, but Id1 was expressed at a higher level in the more aggressive ACCM cell line in comparison to ACC2 cells as confirmed by Id1 promoter-reporter assays. We therefore focused on the ACCM cells for the remainder of the study. We found that proliferation and invasiveness of ACCM cells were strongly reduced after Id1 knockdown whereas Id2 suppression had only a slight effect. Results of scratch and colony formation assays also confirmed that ACCM cell aggressiveness was significantly reduced upon Id1 knockdown. Finally, this knockdown resulted in reduced c-myc and enhanced cyclin-dependent kinase inhibitor p21 expression.
conclusionsThese results demonstrate that Id1 plays an important role in the control of human SGC cell aggressiveness and suggest a potential role as a marker of diagnosis, prognosis and progression of SGCs. Id1 suppression could represent a novel and effective approach for the treatment of salivary gland cancer.
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