ArticleThe Journal of cell biology1990
Protein synthesis and the cell cycle: centrosome reproduction in sea urchin eggs is not under translational control.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 106 citations in OpenAlex.
- The Centriole Biogenesis Cycle: Temporal Mechanisms, Autonomy and Coupling to the Cell Cycle and Other Cellular Clocks.Advances in experimental medicine and biology · 2026Review
- Principles, mechanisms and functions of entrainment in biological oscillators.Interface focus · 2022Review
- Article
- Uncoupling of DNA Replication and Centrosome Duplication Cycles Is a Primary Cause of Haploid Instability in Mammalian Somatic Cells.Frontiers in cell and developmental biology · 2020Article
- Review
- Depletion of Amyloid Precursor Protein (APP) causes G0 arrest in non-small cell lung cancer (NSCLC) cells.Journal of cellular physiology · 2015Article
- One to only two: a short history of the centrosome and its duplication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Loss of cyclin-dependent kinase 2 (CDK2) inhibitory phosphorylation in a CDK2AF knock-in mouse causes misregulation of DNA replication and centrosome duplication.Molecular and cellular biology · 2012Article
- Frequency control of cell cycle oscillators.Current opinion in genetics & development · 2010Review
- Centriole duplication: analogue control in a digital age.Cell biology international · 2010Review
- Centrosome biogenesis continues in the absence of microtubules during prolonged S-phase arrest.Journal of cellular physiology · 2010Article
- Article
- Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1.Journal of cell science · 2010Article
- Cyclin E in centrosome duplication and reduplication in sea urchin zygotes.Journal of cellular physiology · 2008Article
- Centrosome duplication proceeds during mimosine-induced G1 cell cycle arrest.Journal of cellular physiology · 2008Article
- RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycle.Current biology : CB · 2008Article
- Centrosome amplification induced by DNA damage occurs during a prolonged G2 phase and involves ATM.The EMBO journal · 2004Article
- De novo formation of centrosomes in vertebrate cells arrested during S phase.The Journal of cell biology · 2002Article
- Two for two: Cdk2 and its role in centrosome doubling.Oncogene · 2002Review
- Centrosome replication, genomic instability and cancer.Leukemia · 2002Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The reproduction, or duplication, of the centrosome is an important event in a cell's preparation for mitosis. We sought to determine if centrosome reproduction is regulated by the synthesis and accumulation of cyclin proteins and/or the synthesis of centrosome-specific proteins at each cell cycle. We continuously treat sea urchin eggs, starting before fertilization, with a combination of emetine and anisomycin, drugs that have separate targets in the protein synthetic pathway. These drugs inhibit the postfertilization incorporation of [35S]methionine into precipitable material by 97.3-100%. Autoradiography of SDS-PAGE gels of drug-treated zygotes reveals that [35S]methionine incorporates exclusively into material that does not enter the gel and material that runs at the dye front; no other labeled bands are detected. Fertilization events and syngamy are normal in drug-treated zygotes, but the cell cycle arrests before first mitosis. The sperm aster doubles once in all zygotes to yield two asters. In a variable but significant percentage of zygotes, the asters continue to double. This continued doubling is slower than normal, asynchronous between zygotes, and sometimes asynchronous within individual zygotes. High voltage electron microscopy of serial semithick sections from drug-treated zygotes reveals that 90% of the daughter centrosomes contain two centrioles of normal appearance. From these results, we conclude that centrosome reproduction in sea urchin zygotes is not controlled by the accumulation of cyclin proteins or the synthesis of centrosome-specific proteins at each cell cycle. New centrosomes are assembled from preexisting pools of ready-to-use subunits. Furthermore, our results indicate that centrosomal and nuclear events are regulated by separate pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.