ArticlePLoS neglected tropical diseases2013
Characterization of Phlebotomus papatasi peritrophins, and the role of PpPer1 in Leishmania major survival in its natural vector.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Cell wall components of gut commensal bacteria stimulate peritrophic matrix formation in malaria vector mosquitoes through activation of the IMD pathway.PLoS biology · 2025Article
- Genomic analysis of two phlebotomine sand fly vectors of Leishmania from the New and Old World.PLoS neglected tropical diseases · 2023Article
- The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.Parasites & vectors · 2021Article
- Identification of Peritrophins and Antiviral Effect ofInternational journal of molecular sciences · 2020Article
- Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence inmSphere · 2020Article
- Leishmania infection induces a limited differential gene expression in the sand fly midgut.BMC genomics · 2020Article
- C-type lectin-mediated microbial homeostasis is critical for Helicoverpa armigera larval growth and development.PLoS pathogens · 2020Article
- Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions.Frontiers in microbiology · 2020Article
- Tsetse peritrophic matrix influences for trypanosome transmission.Journal of insect physiology · 2019Review
- Refractoriness of Sergentomyia schwetzi to Leishmania spp. is mediated by the peritrophic matrix.PLoS neglected tropical diseases · 2018Article
- A fine-tuned vector-parasite dialogue in tsetse's cardia determines peritrophic matrix integrity and trypanosome transmission success.PLoS pathogens · 2018Article
- PGRP-LD mediates A. stephensi vector competency by regulating homeostasis of microbiota-induced peritrophic matrix synthesis.PLoS pathogens · 2018Article
- VSG overcomes an early barrier to survival of African trypanosomes in tsetse flies.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness.Memorias do Instituto Oswaldo Cruz · 2014Article
- The peritrophic matrix mediates differential infection outcomes in the tsetse fly gut following challenge with commensal, pathogenic, and parasitic microbes.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- An investigation into the protein composition of the teneral Glossina morsitans morsitans peritrophic matrix.PLoS neglected tropical diseases · 2014Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The peritrophic matrix (PM) plays a key role in compartmentalization of the blood meal and as barrier to pathogens in many disease vectors. To establish an infection in sand flies, Leishmania must escape from the endoperitrophic space to prevent excretion with remnants of the blood meal digestion. In spite of the role played regarding Leishmania survival, little is known about sand fly PM molecular components and structural organization. We characterized three peritrophins (PpPer1, PpPer2, and PpPer3) from Phlebotomus papatasi. PpPer1 and PpPer2 display, respectively, four and one chitin-binding domains (CBDs). PpPer3 on the other hand has two CBDs, one mucin-like domain, and a putative domain with hallmarks of a CBD, but with changes in key amino acids. Temporal and spatial expression analyses show that PpPer1 is expressed specifically in the female midgut after blood feeding. PpPer2 and PpPer3 mRNAs were constitutively expressed in midgut and hindgut, with PpPer3 also being expressed in Malpighian tubules. PpPer2 was the only gene expressed in developmental stages. Interestingly, PpPer1 and PpPer3 expression are regulated by Le. major infection. Recombinant PpPer1, PpPer2 and PpPer3 were obtained and shown to display similar biochemical profiles as the native; we also show that PpPer1 and PpPer2 are able to bind chitin. Knockdown of PpPer1 led to a 44% reduction in protein, which in spite of producing an effect on the percentage of infected sand flies, resulted in a 39% increase of parasite load at 48 h. Our data suggest that PpPer1 is a component for the P. papatasi PM and likely involved in the PM role as barrier against Le. major infection.
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